Protein : Qrob_P0371500.2 Q. robur
Protein Identifier  ? Qrob_P0371500.2 Organism . Name  Quercus robur
Score  100.0 Score Type  egn
Protein Description  (M=1) PTHR10631:SF3 - TRNA (GUANINE(26)-N(2))-DIMETHYLTRANSFERASE (PTHR10631:SF3) Code Enzyme  EC:2.1.1.216
Gene Prediction Quality  validated Protein length 

Sequence

Length: 577  
Kegg Orthology  K00555
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0 Synonyms

3 GO Terms

Identifier Name Description
GO:0003723 RNA binding Interacting selectively and non-covalently with an RNA molecule or a portion thereof.
GO:0008033 tRNA processing The process in which a pre-tRNA molecule is converted to a mature tRNA, ready for addition of an aminoacyl group.
GO:0004809 tRNA (guanine-N2-)-methyltransferase activity Catalysis of the reaction: S-adenosyl-L-methionine + tRNA containing guanine = S-adenosyl-L-homocysteine + tRNA containing N2-methylguanine.

32 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|pop:POPTR_0004s11710g 1 576 + 576 Gaps:6 100.00 582 81.62 0.0 POPTRDRAFT_1076440 hypothetical protein
blastp_kegg lcl|vvi:100242902 1 576 + 576 Gaps:3 100.00 579 82.90 0.0 probable N(2) N(2)-dimethylguanosine tRNA methyltransferase 2-like
blastp_kegg lcl|pmum:103329574 1 575 + 575 Gaps:4 100.00 579 81.69 0.0 probable tRNA (guanine(26)-N(2))-dimethyltransferase 2
blastp_kegg lcl|pper:PRUPE_ppa003386mg 1 574 + 574 Gaps:3 99.65 579 81.98 0.0 hypothetical protein
blastp_kegg lcl|tcc:TCM_018962 1 571 + 571 Gaps:3 98.45 581 83.22 0.0 N2 N2-dimethylguanosine tRNA methyltransferase
blastp_kegg lcl|pop:POPTR_0017s13360g 4 573 + 570 Gaps:3 98.28 583 82.02 0.0 hypothetical protein
blastp_kegg lcl|pxb:103962738 1 571 + 571 Gaps:3 98.12 585 81.18 0.0 probable tRNA (guanine(26)-N(2))-dimethyltransferase 2
blastp_kegg lcl|mdm:103404653 1 574 + 574 Gaps:3 99.65 579 80.42 0.0 probable tRNA (guanine(26)-N(2))-dimethyltransferase 2
blastp_kegg lcl|pxb:103926582 1 574 + 574 Gaps:3 99.65 579 80.24 0.0 probable tRNA (guanine(26)-N(2))-dimethyltransferase 2
blastp_kegg lcl|mdm:103442444 1 574 + 574 Gaps:3 99.65 579 81.11 0.0 probable tRNA (guanine(26)-N(2))-dimethyltransferase 2
blastp_pdb 2ytz_B 124 471 + 348 Gaps:47 87.57 378 33.53 4e-42 mol:protein length:378 N(2) N(2)-dimethylguanosine tRNA methyltransf
blastp_pdb 2ytz_A 124 471 + 348 Gaps:47 87.57 378 33.53 4e-42 mol:protein length:378 N(2) N(2)-dimethylguanosine tRNA methyltransf
blastp_pdb 2eju_A 124 471 + 348 Gaps:47 87.57 378 33.53 4e-42 mol:protein length:378 N(2) N(2)-dimethylguanosine tRNA methyltransf
blastp_pdb 2ejt_A 124 471 + 348 Gaps:47 87.57 378 33.53 4e-42 mol:protein length:378 N(2) N(2)-dimethylguanosine tRNA methyltransf
blastp_pdb 2dul_A 124 471 + 348 Gaps:47 87.57 378 33.53 4e-42 mol:protein length:378 N(2) N(2)-dimethylguanosine tRNA methyltransf
blastp_pdb 3axt_A 125 417 + 293 Gaps:25 70.41 392 29.35 2e-30 mol:protein length:392 Probable N(2) N(2)-dimethylguanosine tRNA met
blastp_pdb 3axs_A 125 417 + 293 Gaps:25 70.41 392 29.35 2e-30 mol:protein length:392 Probable N(2) N(2)-dimethylguanosine tRNA met
blastp_uniprot_sprot sp|Q9SRU7|TRM2_ARATH 1 553 + 553 Gaps:9 93.04 589 76.64 0.0 Probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 OS Arabidopsis thaliana GN At3g02320 PE 2 SV 3
blastp_uniprot_sprot sp|Q9LFU5|TRM1_ARATH 1 557 + 557 Gaps:9 93.09 593 75.18 0.0 Probable tRNA (guanine(26)-N(2))-dimethyltransferase 1 OS Arabidopsis thaliana GN At5g15810 PE 2 SV 3
blastp_uniprot_sprot sp|Q3TX08|TRM1_MOUSE 3 534 + 532 Gaps:37 77.38 663 43.86 1e-132 tRNA (guanine(26)-N(2))-dimethyltransferase OS Mus musculus GN Trmt1 PE 1 SV 2
blastp_uniprot_sprot sp|Q9VK89|TRM1_DROME 9 534 + 526 Gaps:49 86.68 578 44.71 7e-128 Probable tRNA (guanine(26)-N(2))-dimethyltransferase OS Drosophila melanogaster GN CG6388 PE 2 SV 1
blastp_uniprot_sprot sp|Q9NXH9|TRM1_HUMAN 9 534 + 526 Gaps:35 77.54 659 42.27 1e-127 tRNA (guanine(26)-N(2))-dimethyltransferase OS Homo sapiens GN TRMT1 PE 1 SV 1
blastp_uniprot_sprot sp|Q9P804|TRM1_SCHPO 11 541 + 531 Gaps:33 93.43 548 36.72 3e-103 tRNA (guanine(26)-N(2))-dimethyltransferase OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN trm1 PE 1 SV 1
blastp_uniprot_sprot sp|Q23270|TRM1_CAEEL 2 540 + 539 Gaps:67 95.06 526 39.60 1e-101 tRNA (guanine(26)-N(2))-dimethyltransferase OS Caenorhabditis elegans GN trm-1 PE 1 SV 1
blastp_uniprot_sprot sp|P15565|TRM1_YEAST 6 533 + 528 Gaps:59 92.46 570 33.02 4e-80 tRNA (guanine(26)-N(2))-dimethyltransferase mitochondrial OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN TRM1 PE 1 SV 1
blastp_uniprot_sprot sp|Q2NI56|TRM1_METST 129 469 + 341 Gaps:24 83.33 390 30.15 6e-48 tRNA (guanine(26)-N(2))-dimethyltransferase OS Methanosphaera stadtmanae (strain ATCC 43021 / DSM 3091 / JCM 11832 / MCB-3) GN trm1 PE 3 SV 1
blastp_uniprot_sprot sp|O27258|TRM1_METTH 129 470 + 342 Gaps:26 84.21 380 30.63 2e-47 tRNA (guanine(26)-N(2))-dimethyltransferase OS Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) GN trm1 PE 3 SV 1
rpsblast_cdd gnl|CDD|145274 126 471 + 346 Gaps:20 86.93 375 44.17 9e-98 pfam02005 TRM N2 N2-dimethylguanosine tRNA methyltransferase. This enzyme EC:2.1.1.32 used S-AdoMet to methylate tRNA. The TRM1 gene of Saccharomyces cerevisiae is necessary for the N2 N2-dimethylguanosine modification of both mitochondrial and cytoplasmic tRNAs. The enzyme is found in both eukaryotes and archaebacteria.
rpsblast_cdd gnl|CDD|32052 119 477 + 359 Gaps:24 88.16 380 36.12 7e-72 COG1867 TRM1 N2 N2-dimethylguanosine tRNA methyltransferase [Translation ribosomal structure and biogenesis].
rpsblast_cdd gnl|CDD|161812 122 471 + 350 Gaps:21 87.97 374 34.35 1e-67 TIGR00308 TRM1 tRNA(guanine-26 N2-N2) methyltransferase. This enzyme is responsible for two methylations of a characteristic guanine of most tRNA molecules. The activity has been demonstrated for eukaryotic and archaeal proteins which are active when expressed in E. coli a species that lacks this enzyme. At least one Eubacterium Aquifex aeolicus has an ortholog as do all completed archaeal genomes.

10 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
PANTHER 4 79 76 PTHR10631:SF3 none none none
Gene3D 125 233 109 G3DSA:3.40.50.150 none none IPR029063
PANTHER 124 553 430 PTHR10631:SF3 none none none
PANTHER 124 553 430 PTHR10631 "MetaCyc:PWY-6829";signature_desc=N(2),N(2)-DIMETHYLGUANOSINE TRNA METHYLTRANSFERASE none IPR002905
SUPERFAMILY 8 48 41 SSF53335 none none IPR029063
SUPERFAMILY 126 472 347 SSF53335 none none IPR029063
Pfam 11 471 461 PF02005 "MetaCyc:PWY-6829" N2,N2-dimethylguanosine tRNA methyltransferase IPR002905
ProSiteProfiles 9 470 462 PS51626 "MetaCyc:PWY-6829" Trm1 methyltransferase domain profile. IPR002905
TIGRFAM 11 472 462 TIGR00308 "MetaCyc:PWY-6829" TRM1: N2,N2-dimethylguanosine tRNA methyltransferase IPR002905
PANTHER 4 79 76 PTHR10631 "MetaCyc:PWY-6829";signature_desc=N(2),N(2)-DIMETHYLGUANOSINE TRNA METHYLTRANSFERASE none IPR002905

0 Localization

9 Qtllist

Qtl Name Chromosome Name Linkage Group Prox Marker Dist Marker Position QTL Pos One Pos Two Test Type Test Value R 2
Bourran1_2004_QTL1_peak_Bud_burst_A4 Qrob_Chr01 1 s_1AMVCC_444 s_1BE1VC_321 4 0 31 lod 3,2 7,4
Bourran2_2003_QTL8_peak_Bud_burst_A4 Qrob_Chr01 1 s_1AH7I_610 s_1BE1VC_321 4,6 0 28 lod 3,5 7,4
Bourran2_2007_QTL6_peak_Bud_burst_3P Qrob_Chr01 1 s_1BE1VC_321 s_1A5JRZ_890 5 0 25 lod 2,4 6
Bourran2_2014_nLBD*_A4 Qrob_Chr08 8 v_12498_318 v_12364_308 34,91 16,12 53,62 lod 2,4961 5,2
Bourran2_2014_rEpiBC*_A4 Qrob_Chr08 8 v_12498_318 v_12364_308 35,77 14,11 55,31 lod 2,9413 6,2
Bourran2_2015_nEpiBC_3P Qrob_Chr12 12 s_1B73S5_217 v_7050_211 28,31 26,37 28,45 lod 4.5 11.6
Bourran2_2015_rEpiBC_3P Qrob_Chr08 8 s_A9TNV_543 v_11837_70 9,93 9,83 11,15 lod 3.3 7.3
Bourran2_2002_QTL7_peak_Bud_burst_A4 Qrob_Chr01 1 s_1DFK66_275 s_1AMVCC_444 3,3 0 7,3 lod 9,4 14,8
Bourran2_2014_nSeqBC*_A4 Qrob_Chr01 1 s_1CFE4C_2114 s_1BDNCB_819 15,25 2 28,5 lod 2,9903 8,3

0 Targeting