Protein : Qrob_P0358330.2 Q. robur
Protein Identifier  ? Qrob_P0358330.2 Organism . Name  Quercus robur
Score  91.1 Score Type  egn
Protein Description  (M=3) 3.1.30.1 - Aspergillus nuclease S(1). Code Enzyme  EC:3.1.30.1
Gene Prediction Quality  validated Protein length 

Sequence

Length: 195  
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0 Synonyms

4 GO Terms

Identifier Name Description
GO:0003676 nucleic acid binding Interacting selectively and non-covalently with any nucleic acid.
GO:0016788 hydrolase activity, acting on ester bonds Catalysis of the hydrolysis of any ester bond.
GO:0004519 endonuclease activity Catalysis of the hydrolysis of ester linkages within nucleic acids by creating internal breaks.
GO:0006308 DNA catabolic process The cellular DNA metabolic process resulting in the breakdown of DNA, deoxyribonucleic acid, one of the two main types of nucleic acid, consisting of a long unbranched macromolecule formed from one or two strands of linked deoxyribonucleotides, the 3'-phosphate group of each constituent deoxyribonucleotide being joined in 3',5'-phosphodiester linkage to the 5'-hydroxyl group of the deoxyribose moiety of the next one.

21 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|pmum:103324657 1 173 + 173 Gaps:38 71.04 297 63.03 5e-87 endonuclease 4-like
blastp_kegg lcl|pper:PRUPE_ppa009322mg 1 173 + 173 Gaps:38 71.04 297 63.03 8e-87 hypothetical protein
blastp_kegg lcl|tcc:TCM_029963 1 177 + 177 Gaps:40 71.38 304 62.21 2e-85 Endonuclease 4 isoform 1
blastp_kegg lcl|mdm:103408833 1 173 + 173 Gaps:40 71.00 300 61.50 3e-85 endonuclease 4-like
blastp_kegg lcl|mdm:103434731 1 173 + 173 Gaps:40 71.00 300 61.50 3e-85 endonuclease 4-like
blastp_kegg lcl|cic:CICLE_v10015663mg 2 173 + 172 Gaps:38 56.60 371 62.86 4e-85 hypothetical protein
blastp_kegg lcl|pxb:103966122 1 173 + 173 Gaps:40 71.00 300 61.50 1e-84 endonuclease 4
blastp_kegg lcl|cic:CICLE_v10016137mg 7 173 + 167 Gaps:38 70.45 291 62.44 2e-83 hypothetical protein
blastp_kegg lcl|rcu:RCOM_1062940 3 173 + 171 Gaps:38 70.13 298 63.64 3e-83 Nuclease PA3 putative (EC:3.1.30.1)
blastp_kegg lcl|cit:102622801 7 173 + 167 Gaps:38 70.45 291 61.95 9e-83 endonuclease 4-like
blastp_pdb 1ak0_A 71 172 + 102 Gaps:14 35.56 270 39.58 4e-08 mol:protein length:270 P1 NUCLEASE
blastp_uniprot_sprot sp|F4JJL0|ENDO4_ARATH 1 173 + 173 Gaps:40 71.24 299 53.99 3e-74 Endonuclease 4 OS Arabidopsis thaliana GN ENDO4 PE 3 SV 1
blastp_uniprot_sprot sp|F4JJL3|ENDO5_ARATH 7 172 + 166 Gaps:41 69.93 296 50.24 1e-64 Endonuclease 5 OS Arabidopsis thaliana GN ENDO5 PE 2 SV 1
blastp_uniprot_sprot sp|Q8LDW6|ENDO3_ARATH 1 172 + 172 Gaps:35 70.41 294 49.28 2e-57 Endonuclease 3 OS Arabidopsis thaliana GN ENDO3 PE 2 SV 1
blastp_uniprot_sprot sp|Q9C9G4|ENDO2_ARATH 3 174 + 172 Gaps:37 72.07 290 46.41 7e-56 Endonuclease 2 OS Arabidopsis thaliana GN ENDO2 PE 1 SV 1
blastp_uniprot_sprot sp|Q9SXA6|ENDO1_ARATH 19 173 + 155 Gaps:41 63.61 305 41.75 3e-43 Endonuclease 1 OS Arabidopsis thaliana GN ENDO1 PE 1 SV 1
blastp_uniprot_sprot sp|P24504|NUP3_PENSQ 71 172 + 102 Gaps:14 35.56 270 39.58 1e-07 Nuclease PA3 OS Penicillium sp. PE 1 SV 1
blastp_uniprot_sprot sp|P24289|NUP1_PENCI 71 172 + 102 Gaps:14 35.56 270 39.58 1e-07 Nuclease P1 OS Penicillium citrinum PE 1 SV 1
blastp_uniprot_sprot sp|P24021|NUS1_ASPOR 54 152 + 99 Gaps:16 31.71 287 42.86 2e-07 Nuclease S1 OS Aspergillus oryzae (strain ATCC 42149 / RIB 40) GN nucS PE 1 SV 2
rpsblast_cdd gnl|CDD|202182 25 173 + 149 Gaps:41 71.71 251 41.11 2e-46 pfam02265 S1-P1_nuclease S1/P1 Nuclease. This family contains both S1 and P1 nucleases (EC:3.1.30.1) which cleave RNA and single stranded DNA with no base specificity.
rpsblast_cdd gnl|CDD|211382 25 173 + 149 Gaps:41 72.29 249 33.89 1e-33 cd11010 S1-P1_nuclease S1/P1 nucleases and related enzymes. This family summarizes both S1 and P1 nucleases (EC:3.1.30.1) which cleave RNA and single stranded DNA with no base specificity. S1 nuclease is more active on DNA than RNA. Its reaction products are oligonucleotides or single nucleotides with 5' phosphoryl groups. Although its primary substrate is single-stranded it may also introduce single-stranded breaks in double-stranded DNA or RNA or DNA-RNA hybrids. It is used as a reagent in nuclease protection assays and in removing single stranded tails from DNA molecules to create blunt ended molecules and opening hairpin loops generated during synthesis of double stranded cDNA. P1 nuclease cleaves its substrate at every position yielding nucleoside 5' monophosphates and it does not recognize or act on double-stranded DNA. It is useful at removing single stranded strands hanging off the end of double stranded DNA and at completely cleaving melted DNA for simple DNA composition analysis.

7 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
Pfam 60 173 114 PF02265 none S1/P1 Nuclease IPR003154
Phobius 1 5 5 NON_CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. none
Phobius 27 194 168 CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. none
Phobius 6 26 21 TRANSMEMBRANE none Region of a membrane-bound protein predicted to be embedded in the membrane. none
SUPERFAMILY 25 174 150 SSF48537 none none IPR008947
Gene3D 25 68 44 G3DSA:1.10.575.10 none none IPR008947
Gene3D 69 174 106 G3DSA:1.10.575.10 none none IPR008947

1 Localization

Analysis Start End Length
SignalP_EUK 1 24 23

2 Qtllist

Qtl Name Chromosome Name Linkage Group Prox Marker Dist Marker Position QTL Pos One Pos Two Test Type Test Value R 2
Bourran2_2014_nSecLBD_3P Qrob_Chr08 8 s_1BN2OD_551 s_1B5AYF_599 17,17 0 43,51 lod 1,9229 4,4
Bourran2_2014_rEpiBC_3P Qrob_Chr05 5 s_2GDU0O_517 s_1BT8O3_239 25,81 0 55,45 lod 2,2101 5,5

0 Targeting