Protein : Qrob_P0305100.2 Q. robur

Protein Identifier  ? Qrob_P0305100.2 Organism . Name  Quercus robur
Score  97.1 Score Type  egn
Protein Description  (M=5) K01507 - inorganic pyrophosphatase [EC:3.6.1.1] Code Enzyme  EC:3.6.1.1
Gene Prediction Quality  validated Protein length 

Sequence

Length: 786  
Kegg Orthology  K01507

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0 Synonyms

4 GO Terms

Identifier Name Description
GO:0016020 membrane A lipid bilayer along with all the proteins and protein complexes embedded in it an attached to it.
GO:0004427 inorganic diphosphatase activity Catalysis of the reaction: diphosphate + H(2)O = H(+) + 2 phosphate.
GO:0009678 hydrogen-translocating pyrophosphatase activity Catalysis of the hydrolysis of pyrophosphate, which generates a proton motive force.
GO:0015992 proton transport The directed movement of protons (hydrogen ions) into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.

28 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|pper:PRUPE_ppa001833mg 1 783 + 783 Gaps:26 99.74 759 91.41 0.0 hypothetical protein
blastp_kegg lcl|pmum:103344065 1 783 + 783 Gaps:26 99.74 759 91.28 0.0 pyrophosphate-energized vacuolar membrane proton pump 1-like
blastp_kegg lcl|mdm:103423547 1 783 + 783 Gaps:26 99.74 759 91.02 0.0 pyrophosphate-energized vacuolar membrane proton pump 1
blastp_kegg lcl|pxb:103962309 1 783 + 783 Gaps:26 99.74 759 90.36 0.0 pyrophosphate-energized vacuolar membrane proton pump 1-like
blastp_kegg lcl|fve:101314765 1 783 + 783 Gaps:25 99.74 760 90.50 0.0 pyrophosphate-energized vacuolar membrane proton pump 1-like
blastp_kegg lcl|mdm:103433876 1 783 + 783 Gaps:26 99.74 759 89.83 0.0 pyrophosphate-energized vacuolar membrane proton pump 1-like
blastp_kegg lcl|tcc:TCM_026755 1 785 + 785 Gaps:27 97.31 781 91.71 0.0 Inorganic H pyrophosphatase family protein isoform 1
blastp_kegg lcl|sot:102604491 1 785 + 785 Gaps:24 100.00 761 88.96 0.0 pyrophosphate-energized vacuolar membrane proton pump 1-like
blastp_kegg lcl|pop:POPTR_0013s01080g 1 785 + 785 Gaps:28 100.00 757 89.70 0.0 POPTRDRAFT_823575 inorganic pyrophosphatase family protein
blastp_kegg lcl|pop:POPTR_0005s01840g 1 783 + 783 Gaps:28 99.74 757 90.07 0.0 inorganic pyrophosphatase family protein
blastp_pdb 4a01_B 9 785 + 777 Gaps:33 98.69 766 83.33 0.0 mol:protein length:766 PROTON PYROPHOSPHATASE
blastp_pdb 4a01_A 9 785 + 777 Gaps:33 98.69 766 83.33 0.0 mol:protein length:766 PROTON PYROPHOSPHATASE
blastp_uniprot_sprot sp|P31414|AVP1_ARATH 3 785 + 783 Gaps:36 99.35 770 82.48 0.0 Pyrophosphate-energized vacuolar membrane proton pump 1 OS Arabidopsis thaliana GN AVP1 PE 1 SV 1
blastp_uniprot_sprot sp|P21616|AVP_VIGRR 9 785 + 777 Gaps:34 98.69 765 83.31 0.0 Pyrophosphate-energized vacuolar membrane proton pump OS Vigna radiata var. radiata PE 1 SV 3
blastp_uniprot_sprot sp|Q06572|AVP_HORVU 1 783 + 783 Gaps:45 99.74 762 81.84 0.0 Pyrophosphate-energized vacuolar membrane proton pump OS Hordeum vulgare PE 2 SV 2
blastp_uniprot_sprot sp|Q8F641|HPPA_LEPIN 52 785 + 734 Gaps:68 94.89 704 58.98 0.0 Putative K(+)-stimulated pyrophosphate-energized sodium pump OS Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601) GN hppA PE 3 SV 1
blastp_uniprot_sprot sp|Q72Q29|HPPA_LEPIC 52 785 + 734 Gaps:68 94.89 704 58.98 0.0 Putative K(+)-stimulated pyrophosphate-energized sodium pump OS Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni (strain Fiocruz L1-130) GN hppA PE 3 SV 1
blastp_uniprot_sprot sp|Q898Q9|HPPA_CLOTE 68 784 + 717 Gaps:96 94.65 673 54.32 0.0 Putative K(+)-stimulated pyrophosphate-energized sodium pump OS Clostridium tetani (strain Massachusetts / E88) GN hppA PE 3 SV 1
blastp_uniprot_sprot sp|Q2RIS7|HPPA1_MOOTA 68 774 + 707 Gaps:73 94.94 672 52.98 0.0 Putative K(+)-stimulated pyrophosphate-energized sodium pump OS Moorella thermoacetica (strain ATCC 39073) GN hppA1 PE 1 SV 1
blastp_uniprot_sprot sp|Q8PYZ8|HPPA1_METMA 12 774 + 763 Gaps:102 98.96 676 51.27 0.0 K(+)-stimulated pyrophosphate-energized sodium pump OS Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) GN hppA1 PE 1 SV 1
blastp_uniprot_sprot sp|Q8TJA9|HPPA1_METAC 12 774 + 763 Gaps:106 98.96 676 51.27 0.0 Putative K(+)-stimulated pyrophosphate-energized sodium pump OS Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A) GN hppA1 PE 3 SV 2
blastp_uniprot_sprot sp|Q3AFC6|HPPA_CARHZ 68 774 + 707 Gaps:90 94.31 686 48.53 2e-173 Putative K(+)-stimulated pyrophosphate-energized sodium pump OS Carboxydothermus hydrogenoformans (strain Z-2901 / DSM 6008) GN hppA PE 1 SV 1
rpsblast_cdd gnl|CDD|177897 1 785 + 785 Gaps:34 100.00 765 85.62 0.0 PLN02255 PLN02255 H(+) -translocating inorganic pyrophosphatase.
rpsblast_cdd gnl|CDD|130174 8 781 + 774 Gaps:78 99.86 697 80.89 0.0 TIGR01104 V_PPase vacuolar-type H(+)-translocating pyrophosphatase. This model describes proton pyrophosphatases from eukaryotes (predominantly plants) archaea and bacteria. It is an integral membrane protein and is suggested to have about 15 membrane spanning domains. Proton translocating inorganic pyrophosphatase like H(+)-ATPase acidifies the vacuoles and is pivotal to the vacuolar secondary active transport systems in plants.
rpsblast_cdd gnl|CDD|202513 17 771 + 755 Gaps:104 100.00 669 54.86 0.0 pfam03030 H_PPase Inorganic H+ pyrophosphatase. The H+ pyrophosphatase is an transmembrane proton pump involved in establishing the H+ electrochemical potential difference between the vacuole lumen and the cell cytosol. Vacuolar-type H(+)-translocating inorganic pyrophosphatases have long been considered to be restricted to plants and to a few species of photo-trophic bacteria. However in recent investigations these pyrophosphatases have been found in organisms as disparate as thermophilic Archaea and parasitic protists.
rpsblast_cdd gnl|CDD|179102 71 779 + 709 Gaps:78 98.05 666 51.30 0.0 PRK00733 hppA membrane-bound proton-translocating pyrophosphatase Validated.
rpsblast_cdd gnl|CDD|33602 71 775 + 705 Gaps:79 94.17 703 48.49 1e-169 COG3808 OVP1 Inorganic pyrophosphatase [Energy production and conversion].
rpsblast_cdd gnl|CDD|177916 70 774 + 705 Gaps:95 96.16 730 41.60 1e-137 PLN02277 PLN02277 H(+) -translocating inorganic pyrophosphatase.

34 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
Pfam 19 771 753 PF03030 "KEGG:00190+3.6.1.1" Inorganic H+ pyrophosphatase IPR004131
Phobius 360 378 19 TRANSMEMBRANE none Region of a membrane-bound protein predicted to be embedded in the membrane. none
Phobius 1 11 11 NON_CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. none
Phobius 783 785 3 CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. none
Phobius 468 472 5 NON_CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. none
Phobius 585 642 58 CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. none
Phobius 12 31 20 TRANSMEMBRANE none Region of a membrane-bound protein predicted to be embedded in the membrane. none
Phobius 538 560 23 TRANSMEMBRANE none Region of a membrane-bound protein predicted to be embedded in the membrane. none
Phobius 495 537 43 CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. none
PIRSF 11 783 773 PIRSF001265 "KEGG:00190+3.6.1.1" none IPR004131
Phobius 205 318 114 NON_CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. none
Phobius 111 129 19 NON_CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. none
Phobius 666 757 92 NON_CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. none
Phobius 422 441 20 CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. none
Phobius 177 204 28 TRANSMEMBRANE none Region of a membrane-bound protein predicted to be embedded in the membrane. none
Phobius 758 782 25 TRANSMEMBRANE none Region of a membrane-bound protein predicted to be embedded in the membrane. none
Phobius 319 340 22 TRANSMEMBRANE none Region of a membrane-bound protein predicted to be embedded in the membrane. none
PANTHER 1 695 695 PTHR31998:SF4 none none none
PANTHER 725 785 61 PTHR31998:SF4 none none none
Phobius 379 397 19 NON_CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. none
Phobius 561 565 5 NON_CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. none
Phobius 473 494 22 TRANSMEMBRANE none Region of a membrane-bound protein predicted to be embedded in the membrane. none
Phobius 130 156 27 TRANSMEMBRANE none Region of a membrane-bound protein predicted to be embedded in the membrane. none
Phobius 643 665 23 TRANSMEMBRANE none Region of a membrane-bound protein predicted to be embedded in the membrane. none
Phobius 90 110 21 TRANSMEMBRANE none Region of a membrane-bound protein predicted to be embedded in the membrane. none
PANTHER 1 695 695 PTHR31998 none none none
PANTHER 725 785 61 PTHR31998 none none none
TIGRFAM 9 695 687 TIGR01104 "KEGG:00190+3.6.1.1" V_PPase: V-type H(+)-translocating pyrophosphatase IPR004131
Phobius 566 584 19 TRANSMEMBRANE none Region of a membrane-bound protein predicted to be embedded in the membrane. none
Phobius 398 421 24 TRANSMEMBRANE none Region of a membrane-bound protein predicted to be embedded in the membrane. none

12 Localization

Analysis Start End Length
TMHMM 360 382 22
TMHMM 758 780 22
TMHMM 10 32 22
TMHMM 182 204 22
TMHMM 90 112 22
TMHMM 570 587 17
TMHMM 472 494 22
TMHMM 445 467 22
TMHMM 132 154 22
TMHMM 318 340 22
TMHMM 397 416 19
TMHMM 538 560 22

6 Qtllist

Qtl Name Chromosome Name Linkage Group Prox Marker Dist Marker Position QTL Pos One Pos Two Test Type Test Value R 2
Bourran2_2014_nLBD*_A4 Qrob_Chr08 8 v_12498_318 v_12364_308 34,91 16,12 53,62 lod 2,4961 5,2
Bourran2_2014_rEpiBC*_A4 Qrob_Chr08 8 v_12498_318 v_12364_308 35,77 14,11 55,31 lod 2,9413 6,2
Bourran_2000_2002_QTL6_Delta.F Qrob_Chr03 3 s_2HYXJ2_207 s_1AUAXK_317 29.12 6,3 45,37 lod 3.1666 0.026
Bourran2_2014_nLBD_3P Qrob_Chr03 3 s_1ET7H8_604 s_1BYNB5_834 23,73 0 46,72 lod 1,7573 3,8
PM_1999_QTL14_peak_Bud_burst_3P Qrob_Chr03 3 s_2F0SI1_756 v_6056_735 11,78 3,78 50,78 lod 2,5 4,5
Bourran2_2014_rEpiBC*_3P Qrob_Chr03 3 s_1A3B3X_1163 s_1DKGMO_450 23,04 4,92 41,12 lod 2,5668 7

1 Targeting

Analysis Start End Length Location Reliability Signal Peptide Cut Off Mitochondrion Cut Off Network Signal Peptide Length
TargetP 1 24   Secretory pathway 1 0.966 0.023 NON-PLANT 24