Protein : Qrob_P0305090.2 Q. robur
Protein Identifier  ? Qrob_P0305090.2 Organism . Name  Quercus robur
Score  100.0 Score Type  egn
Protein Description  (M=1) PTHR10436//PTHR10436:SF53 - RNA PSEUDOURIDYLATE SYNTHASE FAMILY PROTEIN // SUBFAMILY NOT NAMED Code Enzyme  EC:5.4.99.24
Gene Prediction Quality  validated Protein length 

Sequence

Length: 347  
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0 Synonyms

4 GO Terms

Identifier Name Description
GO:0003723 RNA binding Interacting selectively and non-covalently with an RNA molecule or a portion thereof.
GO:0001522 pseudouridine synthesis The intramolecular conversion of uridine to pseudouridine within an RNA molecule. This posttranscriptional base modification occurs in tRNA, rRNA, and snRNAs.
GO:0009451 RNA modification The covalent alteration of one or more nucleotides within an RNA molecule to produce an RNA molecule with a sequence that differs from that coded genetically.
GO:0009982 pseudouridine synthase activity Catalysis of the reaction: RNA uridine = RNA pseudouridine. Conversion of uridine in an RNA molecule to pseudouridine by rotation of the C1'-N-1 glycosidic bond of uridine in RNA to a C1'-C5.

39 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|mdm:103423546 39 346 + 308 Gaps:8 89.40 349 75.32 3e-167 RNA pseudouridine synthase 1-like
blastp_kegg lcl|pper:PRUPE_ppa008866mg 41 345 + 305 Gaps:6 97.15 316 77.85 1e-163 hypothetical protein
blastp_kegg lcl|pmum:103344075 41 345 + 305 Gaps:4 97.17 318 76.05 5e-162 RNA pseudouridine synthase 1
blastp_kegg lcl|pxb:103962310 3 346 + 344 Gaps:14 97.99 349 71.05 6e-161 RNA pseudouridine synthase 1-like
blastp_kegg lcl|cit:102618980 40 345 + 306 Gaps:12 97.53 324 70.89 4e-155 RNA pseudouridine synthase 1-like
blastp_kegg lcl|fve:101300268 11 345 + 335 Gaps:15 93.37 347 71.30 1e-154 RNA pseudourine synthase 1-like
blastp_kegg lcl|cic:CICLE_v10001637mg 7 345 + 339 Gaps:20 96.08 357 67.35 2e-154 hypothetical protein
blastp_kegg lcl|cmo:103494301 39 345 + 307 Gaps:6 94.21 328 66.34 2e-148 RNA pseudouridine synthase 1
blastp_kegg lcl|rcu:RCOM_1434890 41 345 + 305 Gaps:8 96.88 321 72.99 2e-147 ribosomal pseudouridine synthase putative (EC:3.2.1.17)
blastp_kegg lcl|csv:101219820 11 345 + 335 Gaps:9 91.26 366 62.57 9e-146 RNA pseudourine synthase 1-like
blastp_pdb 1xpi_B 90 346 + 257 Gaps:51 95.24 231 33.64 2e-16 mol:protein length:231 Ribosomal large subunit pseudouridine synthas
blastp_pdb 1xpi_A 90 346 + 257 Gaps:51 95.24 231 33.64 2e-16 mol:protein length:231 Ribosomal large subunit pseudouridine synthas
blastp_pdb 1v9k_B 90 346 + 257 Gaps:49 96.49 228 32.73 2e-16 mol:protein length:228 Ribosomal large subunit pseudouridine synthas
blastp_pdb 1v9k_A 90 346 + 257 Gaps:49 96.49 228 32.73 2e-16 mol:protein length:228 Ribosomal large subunit pseudouridine synthas
blastp_pdb 1prz_A 89 337 + 249 Gaps:75 86.51 252 27.06 2e-10 mol:protein length:252 Ribosomal large subunit pseudouridine synthas
blastp_pdb 1qyu_A 89 337 + 249 Gaps:65 62.46 349 27.06 1e-09 mol:protein length:349 Ribosomal large subunit pseudouridine synthas
blastp_pdb 2ist_A 89 338 + 250 Gaps:65 67.38 325 26.94 1e-09 mol:protein length:325 Ribosomal large subunit pseudouridine synthas
blastp_pdb 1v9f_A 89 338 + 250 Gaps:65 67.38 325 26.94 1e-09 mol:protein length:325 Ribosomal large subunit pseudouridine synthas
blastp_uniprot_sprot sp|Q2QNM3|PUS1_ORYSJ 66 342 + 277 Gaps:12 80.29 345 62.45 4e-117 RNA pseudouridine synthase 1 OS Oryza sativa subsp. japonica GN Os12g0560500 PE 2 SV 1
blastp_uniprot_sprot sp|Q7XA65|PUS1_ARATH 53 341 + 289 Gaps:13 87.58 322 61.35 2e-109 RNA pseudouridine synthase 1 OS Arabidopsis thaliana GN At1g56345 PE 2 SV 1
blastp_uniprot_sprot sp|Q45480|YLYB_BACSU 89 345 + 257 Gaps:58 71.62 303 35.48 4e-19 Uncharacterized RNA pseudouridine synthase YlyB OS Bacillus subtilis (strain 168) GN ylyB PE 3 SV 3
blastp_uniprot_sprot sp|P44433|RLUC_HAEIN 90 346 + 257 Gaps:47 67.70 322 33.94 1e-18 Ribosomal large subunit pseudouridine synthase C OS Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) GN rluC PE 3 SV 1
blastp_uniprot_sprot sp|Q8DEV0|RLUD_VIBVU 89 338 + 250 Gaps:67 67.38 325 31.96 5e-18 Ribosomal large subunit pseudouridine synthase D OS Vibrio vulnificus (strain CMCP6) GN rluD PE 3 SV 1
blastp_uniprot_sprot sp|Q9KU20|RLUD_VIBCH 89 337 + 249 Gaps:61 67.28 324 33.03 5e-17 Ribosomal large subunit pseudouridine synthase D OS Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) GN rluD PE 3 SV 1
blastp_uniprot_sprot sp|O31613|YJBO_BACSU 79 345 + 267 Gaps:55 79.15 283 36.16 7e-17 Uncharacterized RNA pseudouridine synthase YjbO OS Bacillus subtilis (strain 168) GN yjbO PE 3 SV 1
blastp_uniprot_sprot sp|Q89AH2|RLUC_BUCBP 52 346 + 295 Gaps:71 81.90 315 31.78 7e-17 Ribosomal large subunit pseudouridine synthase C OS Buchnera aphidicola subsp. Baizongia pistaciae (strain Bp) GN rluC PE 3 SV 1
blastp_uniprot_sprot sp|Q87S65|RLUD_VIBPA 89 338 + 250 Gaps:65 67.38 325 33.79 8e-17 Ribosomal large subunit pseudouridine synthase D OS Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633) GN rluD PE 3 SV 1
blastp_uniprot_sprot sp|O50310|Y723_CHLP8 90 346 + 257 Gaps:68 73.09 327 30.96 2e-16 Uncharacterized RNA pseudouridine synthase Cpar_0723 OS Chlorobaculum parvum (strain NCIB 8327) GN Cpar_0723 PE 3 SV 1
rpsblast_cdd gnl|CDD|30910 51 337 + 287 Gaps:64 79.93 289 33.77 1e-38 COG0564 RluA Pseudouridylate synthases 23S RNA-specific [Translation ribosomal structure and biogenesis].
rpsblast_cdd gnl|CDD|211346 97 321 + 225 Gaps:48 100.00 185 37.30 4e-34 cd02869 PseudoU_synth_RluCD_like Pseudouridine synthase RsuA/RluD family. This group is comprised of eukaryotic bacterial and archeal proteins similar to eight site specific Escherichia coli pseudouridine synthases: RsuA RluA RluB RluC RluD RluE RluF and TruA. Pseudouridine synthases catalyze the isomerization of specific uridines in a n RNA molecule to pseudouridines (5-ribosyluracil psi) requiring no cofactors. E. coli RluC for example makes psi955 2504 and 2580 in 23S RNA. Some psi sites such as psi1917 in 23S RNA made by RluD are universally conserved. Other psi sites occur in a more restricted fashion for example psi2819 in 21S mitochondrial ribosomal RNA made by S. cerevisiae Pus5p is only found in mitochondrial large subunit rRNAs from some other species and in gram negative bacteria. The E. coli counterpart of this psi residue is psi2580 in 23S rRNA. psi2604in 23S RNA made by RluF has only been detected in E.coli.

8 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
PANTHER 58 233 176 PTHR10436:SF53 none none none
SUPERFAMILY 263 345 83 SSF55120 none none IPR020103
SUPERFAMILY 88 230 143 SSF55120 none none IPR020103
PANTHER 264 342 79 PTHR10436 none none none
Pfam 97 286 190 PF00849 none RNA pseudouridylate synthase IPR006145
PANTHER 264 342 79 PTHR10436:SF53 none none none
PANTHER 58 233 176 PTHR10436 none none none
ProSitePatterns 136 150 15 PS01129 none Rlu family of pseudouridine synthase signature. IPR006224

1 Localization

Analysis Start End Length
SignalP_GRAM_POSITIVE 1 36 35

6 Qtllist

Qtl Name Chromosome Name Linkage Group Prox Marker Dist Marker Position QTL Pos One Pos Two Test Type Test Value R 2
Bourran2_2014_nLBD*_A4 Qrob_Chr08 8 v_12498_318 v_12364_308 34,91 16,12 53,62 lod 2,4961 5,2
Bourran2_2014_rEpiBC*_A4 Qrob_Chr08 8 v_12498_318 v_12364_308 35,77 14,11 55,31 lod 2,9413 6,2
Bourran_2000_2002_QTL6_Delta.F Qrob_Chr03 3 s_2HYXJ2_207 s_1AUAXK_317 29.12 6,3 45,37 lod 3.1666 0.026
Bourran2_2014_nLBD_3P Qrob_Chr03 3 s_1ET7H8_604 s_1BYNB5_834 23,73 0 46,72 lod 1,7573 3,8
PM_1999_QTL14_peak_Bud_burst_3P Qrob_Chr03 3 s_2F0SI1_756 v_6056_735 11,78 3,78 50,78 lod 2,5 4,5
Bourran2_2014_rEpiBC*_3P Qrob_Chr03 3 s_1A3B3X_1163 s_1DKGMO_450 23,04 4,92 41,12 lod 2,5668 7

0 Targeting