Protein : Qrob_P0285040.2 Q. robur

Protein Identifier  ? Qrob_P0285040.2 Organism . Name  Quercus robur
Score  100.0 Score Type  egn
Protein Description  (M=1) K12670 - oligosaccharyltransferase complex subunit beta Code Enzyme  EC:2.4.99.18
Gene Prediction Quality  validated Protein length 

Sequence

Length: 184  
Kegg Orthology  K12670

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0 Synonyms

3 GO Terms

Identifier Name Description
GO:0005789 endoplasmic reticulum membrane The lipid bilayer surrounding the endoplasmic reticulum.
GO:0004579 dolichyl-diphosphooligosaccharide-protein glycotransferase activity Catalysis of the reaction: dolichyl diphosphooligosaccharide + protein L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by glycosylamine linkage to protein L-asparagine.
GO:0018279 protein N-linked glycosylation via asparagine The glycosylation of protein via the N4 atom of peptidyl-asparagine forming N4-glycosyl-L-asparagine; the most common form is N-acetylglucosaminyl asparagine; N-acetylgalactosaminyl asparagine and N4 glucosyl asparagine also occur. This modification typically occurs in extracellular peptides with an N-X-(ST) motif. Partial modification has been observed to occur with cysteine, rather than serine or threonine, in the third position; secondary structure features are important, and proline in the second or fourth positions inhibits modification.

22 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|vvi:100250299 1 135 + 135 none 31.03 435 79.26 3e-68 dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit-like
blastp_kegg lcl|cam:101493126 5 139 + 135 none 31.11 434 78.52 2e-67 dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit-like
blastp_kegg lcl|cmo:103498431 1 139 + 139 none 31.95 435 77.70 5e-67 dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit
blastp_kegg lcl|csv:101209991 1 139 + 139 none 31.95 435 76.98 4e-66 dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit-like
blastp_kegg lcl|csv:101210373 1 139 + 139 none 31.95 435 76.98 4e-66 dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit-like
blastp_kegg lcl|gmx:100806848 1 135 + 135 Gaps:4 30.72 433 83.46 3e-65 dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit-like
blastp_kegg lcl|cic:CICLE_v10028484mg 1 135 + 135 Gaps:1 31.19 436 74.26 6e-65 hypothetical protein
blastp_kegg lcl|cit:102618847 1 135 + 135 Gaps:1 31.19 436 74.26 6e-65 dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit-like
blastp_kegg lcl|vvi:100244523 1 135 + 135 none 31.03 435 77.04 4e-64 dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit-like
blastp_kegg lcl|pvu:PHAVU_011G047300g 1 139 + 139 Gaps:4 31.64 433 80.29 7e-64 hypothetical protein
blastp_uniprot_sprot sp|Q6ZLK0|OST48_ORYSJ 25 135 + 111 none 25.28 439 75.68 9e-54 Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit OS Oryza sativa subsp. japonica GN OST48 PE 2 SV 1
blastp_uniprot_sprot sp|Q944K2|OST48_ARATH 13 135 + 123 none 28.15 437 74.80 7e-50 Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit OS Arabidopsis thaliana GN OST48 PE 2 SV 1
blastp_uniprot_sprot sp|Q5R501|OST48_PONAB 27 139 + 113 none 25.74 439 48.67 1e-32 Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit OS Pongo abelii GN DDOST PE 2 SV 2
blastp_uniprot_sprot sp|Q641Y0|OST48_RAT 27 139 + 113 none 25.62 441 48.67 2e-32 Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit OS Rattus norvegicus GN Ddost PE 2 SV 1
blastp_uniprot_sprot sp|P39656|OST48_HUMAN 27 139 + 113 none 24.78 456 48.67 2e-32 Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit OS Homo sapiens GN DDOST PE 1 SV 4
blastp_uniprot_sprot sp|O54734|OST48_MOUSE 27 139 + 113 none 25.62 441 48.67 3e-32 Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit OS Mus musculus GN Ddost PE 1 SV 2
blastp_uniprot_sprot sp|Q29381|OST48_PIG 27 139 + 113 none 25.74 439 49.56 3e-32 Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit OS Sus scrofa GN DDOST PE 1 SV 2
blastp_uniprot_sprot sp|A6QPY0|OST48_BOVIN 27 139 + 113 none 25.74 439 49.56 3e-32 Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit OS Bos taurus GN DDOST PE 2 SV 2
blastp_uniprot_sprot sp|Q05052|OST48_CANFA 22 139 + 118 none 26.52 445 47.46 3e-32 Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit OS Canis familiaris GN DDOST PE 1 SV 1
blastp_uniprot_sprot sp|Q6GNR9|OST48_XENLA 27 139 + 113 none 25.80 438 46.90 4e-32 Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit OS Xenopus laevis GN ddost PE 2 SV 1
rpsblast_cdd gnl|CDD|202601 31 137 + 107 Gaps:1 25.65 421 42.59 8e-30 pfam03345 DDOST_48kD Oligosaccharyltransferase 48 kDa subunit beta. Members of this family are involved in asparagine-linked protein glycosylation. In particular dolichyl-diphosphooligosaccharide-protein glycosyltransferase (DDOST) also known as oligosaccharyltransferase EC:2.4.1.119 transfers the high-mannose sugar GlcNAc(2)-Man(9)-Glc(3) from a dolichol-linked donor to an asparagine acceptor in a consensus Asn-X-Ser/Thr motif. In most eukaryotes the DDOST complex is composed of three subunits which in humans are described as a 48kD subunit ribophorin I and ribophorin II. However the yeast DDOST appears to consist of six subunits (alpha beta gamma delta epsilon zeta). The yeast beta subunit is a 45kD polypeptide previously discovered as the Wbp1 protein with known sequence similarity to the human 48kD subunit and the other orthologues. This family includes the 48kD-like subunits from several eukaryotes it also includes the yeast DDOST beta subunit Wbp1.
rpsblast_kog gnl|CDD|37965 2 139 + 138 Gaps:7 29.57 443 48.85 9e-38 KOG2754 KOG2754 KOG2754 Oligosaccharyltransferase beta subunit [Posttranslational modification protein turnover chaperones].

8 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
Pfam 31 139 109 PF03345 "KEGG:00510+2.4.99.18","KEGG:00513+2.4.99.18","UniPathway:UPA00378" Oligosaccharyltransferase 48 kDa subunit beta IPR005013
PANTHER 1 136 136 PTHR10830 "KEGG:00510+2.4.99.18","KEGG:00513+2.4.99.18","UniPathway:UPA00378";signature_desc=DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE 48 KDA SUBUNIT none IPR005013
Phobius 19 183 165 NON_CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. none
Phobius 4 14 11 SIGNAL_PEPTIDE_H_REGION none Hydrophobic region of a signal peptide. none
Phobius 15 18 4 SIGNAL_PEPTIDE_C_REGION none C-terminal region of a signal peptide. none
PANTHER 1 136 136 PTHR10830:SF0 none none none
Phobius 1 3 3 SIGNAL_PEPTIDE_N_REGION none N-terminal region of a signal peptide. none
Phobius 1 18 18 SIGNAL_PEPTIDE none Signal peptide region none

1 Localization

Analysis Start End Length
SignalP_EUK 1 20 19

5 Qtllist

Qtl Name Chromosome Name Linkage Group Prox Marker Dist Marker Position QTL Pos One Pos Two Test Type Test Value R 2
Bourran2_2015_nEpis_3P Qrob_Chr12 12 s_1AOES6_1466 s_1B0DDG_1094 28,97 28,55 30,1 lod 3.6 8.4
Champenoux_2015_nEpis_3P Qrob_Chr11 11 s_1DG9PM_867 s_1BZ083_1312 26,53 25,47 27,72 lod 4.4 8.9
Bourran2_2002_QTL16_peak_Bud_burst_A4 Qrob_Chr11 11 s_A9OIA_166 s_1C6WBF_114 21,23 2,83 42,83 lod 5,1 7,2
Bourran2_2015_nSeqBC_3P Qrob_Chr11 11 s_1DG9PM_867 s_1BZ083_1312 26,06 25,47 27,72 lod 3.6 7.1
Champenoux_2015_nSecLBD_3P Qrob_Chr11 11 s_1DG9PM_867 s_1BZ083_1312 25,78 25,47 27,72 lod 6.3 16.6

1 Targeting

Analysis Start End Length Location Reliability Signal Peptide Cut Off Mitochondrion Cut Off Network Signal Peptide Length
TargetP 1 20   Secretory pathway 1 0.965 0.034 NON-PLANT 20