Protein : Qrob_P0152230.2 Q. robur

Protein Identifier  ? Qrob_P0152230.2 Organism . Name  Quercus robur
Score  100.0 Score Type  egn
Protein Description  (M=4) 6.1.1.12 - Aspartate--tRNA ligase. Code Enzyme  EC:6.1.1.12
Gene Prediction Quality  validated Protein length 

Sequence

Length: 245  
Kegg Orthology  K01876

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0 Synonyms

8 GO Terms

Identifier Name Description
GO:0003676 nucleic acid binding Interacting selectively and non-covalently with any nucleic acid.
GO:0005524 ATP binding Interacting selectively and non-covalently with ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
GO:0005737 cytoplasm All of the contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
GO:0000166 nucleotide binding Interacting selectively and non-covalently with a nucleotide, any compound consisting of a nucleoside that is esterified with (ortho)phosphate or an oligophosphate at any hydroxyl group on the ribose or deoxyribose.
GO:0004812 aminoacyl-tRNA ligase activity Catalysis of the formation of aminoacyl-tRNA from ATP, amino acid, and tRNA with the release of diphosphate and AMP.
GO:0006418 tRNA aminoacylation for protein translation The synthesis of aminoacyl tRNA by the formation of an ester bond between the 3'-hydroxyl group of the most 3' adenosine of the tRNA, to be used in ribosome-mediated polypeptide synthesis.
GO:0004815 aspartate-tRNA ligase activity Catalysis of the reaction: ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp).
GO:0006422 aspartyl-tRNA aminoacylation The process of coupling aspartate to aspartyl-tRNA, catalyzed by aspartyl-tRNA synthetase. In tRNA aminoacylation, the amino acid is first activated by linkage to AMP and then transferred to either the 2'- or the 3'-hydroxyl group of the 3'-adenosine residue of the tRNA.

37 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|csv:101222217 41 211 + 171 Gaps:2 31.74 545 64.74 1e-66 aspartate--tRNA ligase cytoplasmic-like
blastp_kegg lcl|cmo:103503781 42 211 + 170 Gaps:2 31.56 545 65.12 3e-66 aspartate--tRNA ligase cytoplasmic
blastp_kegg lcl|sot:102590401 1 212 + 212 Gaps:11 38.03 539 58.05 1e-62 aspartate--tRNA ligase cytoplasmic-like
blastp_kegg lcl|pop:POPTR_0006s08550g 3 211 + 209 Gaps:6 38.35 545 65.55 5e-62 POPTRDRAFT_652970 aspartyl-tRNA synthetase family protein
blastp_kegg lcl|mdm:103456132 9 219 + 211 Gaps:7 44.12 485 62.62 7e-62 aspartate--tRNA ligase cytoplasmic-like
blastp_kegg lcl|mdm:103415490 9 219 + 211 Gaps:7 39.27 545 62.62 3e-61 aspartate--tRNA ligase cytoplasmic-like
blastp_kegg lcl|mtr:MTR_1g014140 1 211 + 211 Gaps:9 38.08 541 58.74 4e-58 Aspartyl-tRNA synthetase
blastp_kegg lcl|pxb:103965980 50 219 + 170 Gaps:5 31.55 542 61.99 4e-58 aspartate--tRNA ligase cytoplasmic-like
blastp_kegg lcl|mdm:103441378 48 219 + 172 Gaps:3 31.55 542 61.40 4e-58 aspartate--tRNA ligase cytoplasmic-like
blastp_kegg lcl|rcu:RCOM_1043210 15 212 + 198 Gaps:7 37.96 540 60.49 5e-58 aspartyl-tRNA synthetase putative (EC:6.1.1.12)
blastp_pdb 1asz_B 59 209 + 151 Gaps:3 31.43 490 37.01 7e-17 mol:protein length:490 ASPARTYL-tRNA SYNTHETASE
blastp_pdb 1asz_A 59 209 + 151 Gaps:3 31.43 490 37.01 7e-17 mol:protein length:490 ASPARTYL-tRNA SYNTHETASE
blastp_pdb 1asy_B 59 209 + 151 Gaps:3 31.43 490 37.01 7e-17 mol:protein length:490 ASPARTYL-tRNA SYNTHETASE
blastp_pdb 1asy_A 59 209 + 151 Gaps:3 31.43 490 37.01 7e-17 mol:protein length:490 ASPARTYL-tRNA SYNTHETASE
blastp_pdb 1eov_A 64 209 + 146 Gaps:3 30.60 487 36.91 4e-16 mol:protein length:487 ASPARTYL-TRNA SYNTHETASE
blastp_pdb 3i7f_B 63 198 + 136 Gaps:3 24.64 548 32.59 3e-12 mol:protein length:548 Aspartyl-tRNA synthetase
blastp_pdb 3i7f_A 63 198 + 136 Gaps:3 24.64 548 32.59 3e-12 mol:protein length:548 Aspartyl-tRNA synthetase
blastp_uniprot_sprot sp|Q3SYZ4|SYDC_BOVIN 59 208 + 150 Gaps:5 29.34 501 42.18 1e-20 Aspartate--tRNA ligase cytoplasmic OS Bos taurus GN DARS PE 2 SV 1
blastp_uniprot_sprot sp|P15178|SYDC_RAT 59 208 + 150 Gaps:5 29.34 501 40.82 2e-20 Aspartate--tRNA ligase cytoplasmic OS Rattus norvegicus GN Dars PE 2 SV 1
blastp_uniprot_sprot sp|Q922B2|SYDC_MOUSE 59 208 + 150 Gaps:5 29.34 501 41.50 2e-20 Aspartate--tRNA ligase cytoplasmic OS Mus musculus GN Dars PE 2 SV 2
blastp_uniprot_sprot sp|P04802|SYDC_YEAST 21 209 + 189 Gaps:9 35.55 557 35.35 6e-20 Aspartate--tRNA ligase cytoplasmic OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN DPS1 PE 1 SV 3
blastp_uniprot_sprot sp|Q5R9I5|SYDC_PONAB 59 199 + 141 Gaps:5 27.54 501 42.03 3e-19 Aspartate--tRNA ligase cytoplasmic OS Pongo abelii GN DARS PE 2 SV 1
blastp_uniprot_sprot sp|P14868|SYDC_HUMAN 59 199 + 141 Gaps:5 27.54 501 42.03 7e-19 Aspartate--tRNA ligase cytoplasmic OS Homo sapiens GN DARS PE 1 SV 2
blastp_uniprot_sprot sp|Q03577|SYDC_CAEEL 29 201 + 173 Gaps:10 32.96 531 35.43 6e-18 Aspartate--tRNA ligase cytoplasmic OS Caenorhabditis elegans GN drs-1 PE 3 SV 1
blastp_uniprot_sprot sp|O74407|SYDC_SCHPO 60 209 + 150 Gaps:5 25.69 580 36.91 7e-16 Aspartate--tRNA ligase cytoplasmic OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN dps1 PE 1 SV 1
blastp_uniprot_sprot sp|Q75JQ1|SYDC1_DICDI 65 208 + 144 Gaps:7 24.48 576 36.88 2e-15 Aspartate--tRNA ligase cytoplasmic 1 OS Dictyostelium discoideum GN aspS1 PE 3 SV 1
blastp_uniprot_sprot sp|Q559M9|SYDC2_DICDI 85 201 + 117 Gaps:3 21.09 569 28.33 5e-11 Aspartate--tRNA ligase cytoplasmic 2 OS Dictyostelium discoideum GN aspS2 PE 3 SV 1
rpsblast_cdd gnl|CDD|178442 17 211 + 195 Gaps:3 36.23 530 60.94 2e-70 PLN02850 PLN02850 aspartate-tRNA ligase.
rpsblast_cdd gnl|CDD|58590 99 198 + 100 Gaps:2 100.00 102 47.06 2e-28 cd04320 AspRS_cyto_N AspRS_cyto_N: N-terminal anticodon recognition domain of the type found in Saccharomyces cerevisiae and human cytoplasmic aspartyl-tRNA synthetase (AspRS). This domain is a beta-barrel domain (OB fold) involved in binding the tRNA anticodon stem-loop. The enzymes in this group are homodimeric class2b aminoacyl-tRNA synthetases (aaRSs). aaRSs catalyze the specific attachment of amino acids (AAs) to their cognate tRNAs during protein biosynthesis. This 2-step reaction involves i) the activation of the AA by ATP in the presence of magnesium ions followed by ii) the transfer of the activated AA to the terminal ribose of tRNA. In the case of the class2b aaRSs the activated AA is attached to the 3'OH of the terminal ribose. Eukaryotes contain 2 sets of aaRSs both of which are encoded by the nuclear genome. One set concerns with cytoplasmic protein synthesis whereas the other exclusively with mitochondrial protein synthesis..
rpsblast_cdd gnl|CDD|173592 20 205 + 186 Gaps:10 32.73 550 34.44 7e-21 PTZ00401 PTZ00401 aspartyl-tRNA synthetase Provisional.

6 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
PANTHER 5 213 209 PTHR22594:SF10 none none IPR004523
Gene3D 63 191 129 G3DSA:2.40.50.140 none none IPR012340
SUPERFAMILY 63 191 129 SSF50249 none none IPR012340
PANTHER 5 213 209 PTHR22594 "Reactome:REACT_71";signature_desc=ASPARTYL/LYSYL-TRNA SYNTHETASE none IPR018150
Coils 28 49 22 Coil none none none
Pfam 100 182 83 PF01336 none OB-fold nucleic acid binding domain IPR004365

0 Localization

2 Qtllist

Qtl Name Chromosome Name Linkage Group Prox Marker Dist Marker Position QTL Pos One Pos Two Test Type Test Value R 2
Bourran2_2015_nEpis_3P Qrob_Chr12 12 s_1AOES6_1466 s_1B0DDG_1094 28,97 28,55 30,1 lod 3.6 8.4
Bourran2_2015_nP_3P Qrob_Chr12 12 v_10140_295 v_838_303 16 14,69 18,01 lod 5.1 13.7

0 Targeting