Protein : Qrob_P0146150.2 Q. robur
Protein Identifier  ? Qrob_P0146150.2 Organism . Name  Quercus robur
Score  0.0 Score Type  egn
Protein Description  (M=1) 2.5.1.43 - Nicotianamine synthase. Code Enzyme  EC:2.5.1.43
Gene Prediction Quality  validated Protein length 

Sequence

Length: 171  
Kegg Orthology  K05953
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0 Synonyms

2 GO Terms

Identifier Name Description
GO:0030410 nicotianamine synthase activity Catalysis of the reaction: 3 S-adenosyl-L-methionine(1+) = 3 S-methyl-5'-thioadenosine + 3 H(+) + nicotianamine.
GO:0030418 nicotianamine biosynthetic process The chemical reactions and pathways resulting in the formation of nicotianamine, 2(S),3'2(S),3''(S)-N-(N-(3-amino-3-carboxypropyl)-3-amino-3-carboxypropyl)-azetidine-2-carboxylic acid.

22 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|vvi:100254544 1 170 + 170 Gaps:1 55.52 308 81.29 3e-92 nicotianamine synthase-like
blastp_kegg lcl|pper:PRUPE_ppa008576mg 1 170 + 170 Gaps:1 52.45 326 80.12 5e-91 hypothetical protein
blastp_kegg lcl|pmum:103344131 1 170 + 170 Gaps:1 52.45 326 80.12 5e-91 nicotianamine synthase
blastp_kegg lcl|pop:POPTR_0005s01730g 1 170 + 170 Gaps:1 63.57 269 78.95 5e-90 hypothetical protein
blastp_kegg lcl|rcu:RCOM_1435160 1 170 + 170 Gaps:1 53.44 320 78.95 8e-90 Nicotianamine synthase putative (EC:2.5.1.43)
blastp_kegg lcl|tcc:TCM_026765 1 170 + 170 Gaps:1 54.11 316 77.78 4e-89 Nicotianamine synthase 3
blastp_kegg lcl|fve:101313617 1 170 + 170 Gaps:1 51.66 331 78.36 9e-89 nicotianamine synthase-like
blastp_kegg lcl|pxb:103930560 1 170 + 170 Gaps:1 52.78 324 77.78 5e-88 nicotianamine synthase-like
blastp_kegg lcl|mdm:103433868 1 170 + 170 Gaps:1 52.62 325 77.19 6e-87 nicotianamine synthase-like
blastp_kegg lcl|pxb:103962314 1 170 + 170 Gaps:1 52.62 325 75.44 3e-86 nicotianamine synthase
blastp_uniprot_sprot sp|Q9XGI7|NAS_SOLLC 7 170 + 164 Gaps:1 52.05 317 69.09 4e-77 Nicotianamine synthase OS Solanum lycopersicum GN CHLN PE 2 SV 1
blastp_uniprot_sprot sp|Q9FF79|NAS1_ARATH 1 170 + 170 Gaps:5 54.69 320 62.86 2e-69 Nicotianamine synthase 1 OS Arabidopsis thaliana GN NAS1 PE 2 SV 1
blastp_uniprot_sprot sp|O80483|NAS3_ARATH 1 170 + 170 Gaps:4 54.37 320 64.94 1e-67 Nicotianamine synthase 3 OS Arabidopsis thaliana GN NAS3 PE 2 SV 1
blastp_uniprot_sprot sp|Q9FKT9|NAS2_ARATH 1 170 + 170 Gaps:4 54.37 320 62.64 4e-67 Nicotianamine synthase 2 OS Arabidopsis thaliana GN NAS2 PE 2 SV 1
blastp_uniprot_sprot sp|Q9C7X5|NAS4_ARATH 3 170 + 168 Gaps:4 53.09 324 62.21 9e-67 Probable nicotianamine synthase 4 OS Arabidopsis thaliana GN NAS4 PE 2 SV 1
blastp_uniprot_sprot sp|Q9ZQV6|NAS4_HORVU 1 170 + 170 Gaps:6 53.50 329 51.70 2e-57 Probable nicotianamine synthase 4 OS Hordeum vulgare GN NAS4 PE 2 SV 1
blastp_uniprot_sprot sp|Q9ZQV8|NAS3_HORVU 8 170 + 163 Gaps:2 49.25 335 53.33 2e-57 Probable nicotianamine synthase 3 OS Hordeum vulgare GN NAS3 PE 2 SV 1
blastp_uniprot_sprot sp|Q9XFB6|NAS8_HORVU 1 170 + 170 Gaps:6 53.50 329 51.14 7e-57 Nicotianamine synthase 8 OS Hordeum vulgare GN NAS8 PE 1 SV 1
blastp_uniprot_sprot sp|Q9ZQV7|NAS2_HORVU 6 170 + 165 Gaps:3 50.15 335 50.00 3e-54 Probable nicotianamine synthase 2 OS Hordeum vulgare GN NAS2 PE 2 SV 1
blastp_uniprot_sprot sp|Q9ZWH8|NAS7_HORVU 1 170 + 170 Gaps:6 53.50 329 50.00 4e-54 Probable nicotianamine synthase 7 OS Hordeum vulgare GN NAS7 PE 2 SV 1
rpsblast_cdd gnl|CDD|178624 1 170 + 170 Gaps:1 57.77 296 71.35 4e-83 PLN03075 PLN03075 nicotianamine synthase Provisional.
rpsblast_cdd gnl|CDD|190511 4 170 + 167 Gaps:1 60.65 277 62.50 2e-72 pfam03059 NAS Nicotianamine synthase protein. Nicotianamine synthase EC:2.5.1.43 catalyzes the trimerisation of S-adenosylmethionine to yield one molecule of nicotianamine. Nicotianamine has an important role in plant iron uptake mechanisms. Plants adopt two strategies (termed I and II) of iron acquisition. Strategy I is adopted by all higher plants except graminaceous plants which adopt strategy II. In strategy I plants the role of nicotianamine is not fully determined: possible roles include the formation of more stable complexes with ferrous than with ferric ion which might serve as a sensor of the physiological status of iron within a plant or which might be involved in the transport of iron. In strategy II (graminaceous) plants nicotianamine is the key intermediate (and nicotianamine synthase the key enzyme) in the synthesis of the mugineic family (the only known family in plants) of phytosiderophores. Phytosiderophores are iron chelators whose secretion by the roots is greatly increased in instances of iron deficiency. The 3D structures of five example NAS from Methanothermobacter thermautotrophicus reveal the monomer to consist of a five-helical bundle N-terminal domain on top of a classic Rossmann fold C-terminal domain. The N-terminal domain is unique to the NAS family whereas the C-terminal domain is homologous to the class I family of SAM-dependent methyltransferases. An active site is created at the interface of the two domains at the rim of a large cavity that corresponds to the nucleotide binding site such as is found in other proteins adopting a Rossmann fold.

4 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
Pfam 4 170 167 PF03059 "MetaCyc:PWY-5912","MetaCyc:PWY-5957" Nicotianamine synthase protein IPR004298
PANTHER 1 170 170 PTHR32266:SF9 none none none
ProSiteProfiles 1 170 170 PS51142 "MetaCyc:PWY-5912","MetaCyc:PWY-5957" Nicotianamine synthase (NAS)-like family profile. IPR004298
PANTHER 1 170 170 PTHR32266 none none none

0 Localization

6 Qtllist

Qtl Name Chromosome Name Linkage Group Prox Marker Dist Marker Position QTL Pos One Pos Two Test Type Test Value R 2
Bourran2_2014_nLBD*_A4 Qrob_Chr08 8 v_12498_318 v_12364_308 34,91 16,12 53,62 lod 2,4961 5,2
Bourran2_2014_rEpiBC*_A4 Qrob_Chr08 8 v_12498_318 v_12364_308 35,77 14,11 55,31 lod 2,9413 6,2
Bourran_2000_2002_QTL6_Delta.F Qrob_Chr03 3 s_2HYXJ2_207 s_1AUAXK_317 29.12 6,3 45,37 lod 3.1666 0.026
Bourran2_2014_nLBD_3P Qrob_Chr03 3 s_1ET7H8_604 s_1BYNB5_834 23,73 0 46,72 lod 1,7573 3,8
PM_1999_QTL14_peak_Bud_burst_3P Qrob_Chr03 3 s_2F0SI1_756 v_6056_735 11,78 3,78 50,78 lod 2,5 4,5
Bourran2_2014_rEpiBC*_3P Qrob_Chr03 3 s_1A3B3X_1163 s_1DKGMO_450 23,04 4,92 41,12 lod 2,5668 7

0 Targeting