Protein : Qrob_P0137160.2 Q. robur

Protein Identifier  ? Qrob_P0137160.2 Organism . Name  Quercus robur
Score  97.5 Score Type  egn
Protein Description  (M=2) K01869 - leucyl-tRNA synthetase [EC:6.1.1.4] Code Enzyme  EC:6.1.1.4
Gene Prediction Quality  validated Protein length 

Sequence

Length: 1140  
Kegg Orthology  K01869

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0 Synonyms

8 GO Terms

Identifier Name Description
GO:0005524 ATP binding Interacting selectively and non-covalently with ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
GO:0005737 cytoplasm All of the contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
GO:0000166 nucleotide binding Interacting selectively and non-covalently with a nucleotide, any compound consisting of a nucleoside that is esterified with (ortho)phosphate or an oligophosphate at any hydroxyl group on the ribose or deoxyribose.
GO:0004812 aminoacyl-tRNA ligase activity Catalysis of the formation of aminoacyl-tRNA from ATP, amino acid, and tRNA with the release of diphosphate and AMP.
GO:0006418 tRNA aminoacylation for protein translation The synthesis of aminoacyl tRNA by the formation of an ester bond between the 3'-hydroxyl group of the most 3' adenosine of the tRNA, to be used in ribosome-mediated polypeptide synthesis.
GO:0002161 aminoacyl-tRNA editing activity The hydrolysis of an incorrectly aminoacylated tRNA.
GO:0004823 leucine-tRNA ligase activity Catalysis of the reaction: L-leucine + ATP + tRNA(Leu) = AMP + diphosphate + 2 H(+) + Leu-tRNA(Leu).
GO:0006429 leucyl-tRNA aminoacylation The process of coupling leucine to leucyl-tRNA, catalyzed by leucyl-tRNA synthetase. In tRNA aminoacylation, the amino acid is first activated by linkage to AMP and then transferred to either the 2'- or the 3'-hydroxyl group of the 3'-adenosine residue of the tRNA.

44 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|csv:101231275 34 1118 + 1085 Gaps:4 99.91 1090 83.84 0.0 LARS leucyl-tRNA synthetase
blastp_kegg lcl|cmo:103501922 34 1118 + 1085 Gaps:4 99.91 1090 83.84 0.0 leucine--tRNA ligase cytoplasmic
blastp_kegg lcl|csv:101213618 34 1118 + 1085 Gaps:4 99.91 1090 83.84 0.0 LARS leucyl-tRNA synthetase
blastp_kegg lcl|pmum:103318990 34 1120 + 1087 Gaps:2 100.00 1089 84.39 0.0 leucine--tRNA ligase cytoplasmic
blastp_kegg lcl|tcc:TCM_025952 34 1119 + 1086 Gaps:5 100.00 1089 84.02 0.0 ATP binding leucine-tRNA ligases aminoacyl-tRNA ligases nucleotide binding ATP binding aminoacyl-tRNA ligases
blastp_kegg lcl|cit:102615675 34 1119 + 1086 Gaps:4 100.00 1088 82.90 0.0 leucine--tRNA ligase cytoplasmic-like
blastp_kegg lcl|vvi:100248927 37 1118 + 1082 Gaps:3 99.82 1085 85.60 0.0 leucyl-tRNA synthetase cytoplasmic-like
blastp_kegg lcl|cic:CICLE_v10000088mg 34 1119 + 1086 Gaps:4 100.00 1088 82.90 0.0 hypothetical protein
blastp_kegg lcl|rcu:RCOM_0757640 34 1119 + 1086 Gaps:3 100.00 1087 83.35 0.0 leucyl-tRNA synthetase putative (EC:6.1.1.4)
blastp_kegg lcl|pxb:103946381 34 1120 + 1087 Gaps:12 100.00 1097 83.32 0.0 leucine--tRNA ligase cytoplasmic-like
blastp_pdb 1wz2_B 51 1079 + 1029 Gaps:132 96.48 967 35.80 1e-151 mol:protein length:967 Leucyl-tRNA synthetase
blastp_pdb 1wz2_A 51 1079 + 1029 Gaps:132 96.48 967 35.80 1e-151 mol:protein length:967 Leucyl-tRNA synthetase
blastp_pdb 1wkb_A 51 908 + 858 Gaps:89 98.89 810 37.58 1e-150 mol:protein length:810 Leucyl-tRNA synthetase
blastp_pdb 2wfd_B 298 546 + 249 none 98.81 252 54.22 4e-86 mol:protein length:252 LEUCYL-TRNA SYNTHETASE CYTOPLASMIC
blastp_pdb 2wfd_A 298 546 + 249 none 98.81 252 54.22 4e-86 mol:protein length:252 LEUCYL-TRNA SYNTHETASE CYTOPLASMIC
blastp_pdb 3pz6_F 291 551 + 261 Gaps:54 100.00 311 39.55 3e-57 mol:protein length:311 Leucyl-tRNA synthetase
blastp_pdb 3pz6_E 291 551 + 261 Gaps:54 100.00 311 39.55 3e-57 mol:protein length:311 Leucyl-tRNA synthetase
blastp_pdb 3pz6_D 291 551 + 261 Gaps:54 100.00 311 39.55 3e-57 mol:protein length:311 Leucyl-tRNA synthetase
blastp_pdb 3pz6_C 291 551 + 261 Gaps:54 100.00 311 39.55 3e-57 mol:protein length:311 Leucyl-tRNA synthetase
blastp_pdb 3pz6_B 291 551 + 261 Gaps:54 100.00 311 39.55 3e-57 mol:protein length:311 Leucyl-tRNA synthetase
blastp_uniprot_sprot sp|Q9P2J5|SYLC_HUMAN 40 1136 + 1097 Gaps:38 93.79 1176 50.23 0.0 Leucine--tRNA ligase cytoplasmic OS Homo sapiens GN LARS PE 1 SV 2
blastp_uniprot_sprot sp|Q5R614|SYLC_PONAB 40 1136 + 1097 Gaps:38 93.79 1176 50.05 0.0 Leucine--tRNA ligase cytoplasmic OS Pongo abelii GN LARS PE 2 SV 1
blastp_uniprot_sprot sp|Q8BMJ2|SYLC_MOUSE 34 1139 + 1106 Gaps:25 93.12 1178 49.68 0.0 Leucine--tRNA ligase cytoplasmic OS Mus musculus GN Lars PE 2 SV 2
blastp_uniprot_sprot sp|Q09996|SYLC_CAEEL 47 1120 + 1074 Gaps:44 90.05 1186 48.88 0.0 Leucine--tRNA ligase OS Caenorhabditis elegans GN lrs-1 PE 3 SV 2
blastp_uniprot_sprot sp|Q54N83|SYLC_DICDI 41 1117 + 1077 Gaps:34 99.91 1058 47.59 0.0 Leucine--tRNA ligase cytoplasmic OS Dictyostelium discoideum GN leuS PE 3 SV 1
blastp_uniprot_sprot sp|Q10490|SYLC_SCHPO 44 1116 + 1073 Gaps:65 97.39 1111 46.86 0.0 Putative leucine--tRNA ligase cytoplasmic OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN lrs1 PE 1 SV 1
blastp_uniprot_sprot sp|P10857|SYLC_NEUCR 40 1116 + 1077 Gaps:77 97.60 1123 42.70 0.0 Leucine--tRNA ligase cytoplasmic OS Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) GN leu-6 PE 3 SV 2
blastp_uniprot_sprot sp|P26637|SYLC_YEAST 43 1116 + 1074 Gaps:65 98.81 1090 43.27 0.0 Leucine--tRNA ligase cytoplasmic OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN CDC60 PE 1 SV 1
blastp_uniprot_sprot sp|Q9YD97|SYL_AERPE 46 1020 + 975 Gaps:128 95.62 959 36.64 2e-161 Leucine--tRNA ligase OS Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1) GN leuS PE 3 SV 1
blastp_uniprot_sprot sp|O58698|SYL_PYRHO 51 1079 + 1029 Gaps:132 96.48 967 35.80 4e-151 Leucine--tRNA ligase OS Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) GN leuS PE 1 SV 1

26 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
Pfam 217 788 572 PF00133 "Reactome:REACT_71" tRNA synthetases class I (I, L, M and V) IPR002300
Pfam 56 137 82 PF00133 "Reactome:REACT_71" tRNA synthetases class I (I, L, M and V) IPR002300
ProSitePatterns 87 98 12 PS00178 "Reactome:REACT_71" Aminoacyl-transfer RNA synthetases class-I signature. IPR001412
TIGRFAM 50 1087 1038 TIGR00395 "KEGG:00970+6.1.1.4" leuS_arch: leucine--tRNA ligase IPR004493
Gene3D 563 778 216 G3DSA:3.40.50.620 none none IPR014729
Gene3D 48 141 94 G3DSA:3.40.50.620 none none IPR014729
Gene3D 190 295 106 G3DSA:3.40.50.620 none none IPR014729
SUPERFAMILY 210 291 82 SSF52374 none none none
SUPERFAMILY 49 137 89 SSF52374 none none none
SUPERFAMILY 549 794 246 SSF52374 none none none
Pfam 827 956 130 PF08264 "Reactome:REACT_71" Anticodon-binding domain of tRNA IPR013155
Gene3D 299 562 264 G3DSA:3.90.740.10 "Reactome:REACT_71" none IPR009008
Gene3D 779 924 146 G3DSA:1.10.730.10 "Reactome:REACT_71" none IPR009080
SUPERFAMILY 510 549 40 SSF50677 "Reactome:REACT_71" none IPR009008
SUPERFAMILY 321 470 150 SSF50677 "Reactome:REACT_71" none IPR009008
PANTHER 194 364 171 PTHR11946 none none none
PANTHER 194 364 171 PTHR11946:SF51 none none none
Coils 1062 1083 22 Coil none none none
PANTHER 501 1099 599 PTHR11946:SF51 none none none
Coils 483 504 22 Coil none none none
SUPERFAMILY 820 958 139 SSF47323 "Reactome:REACT_71" none IPR009080
PANTHER 390 448 59 PTHR11946 none none none
PANTHER 501 1099 599 PTHR11946 none none none
PANTHER 43 137 95 PTHR11946 none none none
PANTHER 390 448 59 PTHR11946:SF51 none none none
PANTHER 43 137 95 PTHR11946:SF51 none none none

1 Localization

Analysis Start End Length
TMHMM 3 25 22

1 Qtllist

Qtl Name Chromosome Name Linkage Group Prox Marker Dist Marker Position QTL Pos One Pos Two Test Type Test Value R 2
PM_1999_QTL14_peak_Bud_burst_3P Qrob_Chr03 3 s_2F0SI1_756 v_6056_735 11,78 3,78 50,78 lod 2,5 4,5

1 Targeting

Analysis Start End Length Location Reliability Signal Peptide Cut Off Mitochondrion Cut Off Network Signal Peptide Length
TargetP 1 26   Secretory pathway 1 0.963 0.010 NON-PLANT 26