Protein : Qrob_P0135890.2 Q. robur
Protein Identifier  ? Qrob_P0135890.2 Organism . Name  Quercus robur
Score  100.0 Score Type  egn
Protein Description  (M=25) 3.1.3.2 - Acid phosphatase. Code Enzyme  EC:3.1.3.2
Gene Prediction Quality  validated Protein length 

Sequence

Length: 256  
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0 Synonyms

1 GO Terms

Identifier Name Description
GO:0003993 acid phosphatase activity Catalysis of the reaction: an orthophosphoric monoester + H2O = an alcohol + phosphate, with an acid pH optimum.

26 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|pmum:103334419 1 253 + 253 Gaps:10 99.62 262 81.23 7e-150 acid phosphatase 1-like
blastp_kegg lcl|pper:PRUPE_ppa010275mg 1 253 + 253 Gaps:4 99.61 256 81.57 8e-149 hypothetical protein
blastp_kegg lcl|tcc:TCM_003933 17 255 + 239 none 93.73 255 81.59 1e-145 HAD superfamily subfamily IIIB acid phosphatase
blastp_kegg lcl|pop:POPTR_0002s25950g 1 255 + 255 none 100.00 255 74.51 1e-142 POPTRDRAFT_711542 hypothetical protein
blastp_kegg lcl|rcu:RCOM_0542930 1 251 + 251 Gaps:4 93.75 272 75.69 2e-140 Acid phosphatase 1 precursor putative (EC:3.1.3.2)
blastp_kegg lcl|cic:CICLE_v10021795mg 1 255 + 255 Gaps:1 100.00 256 72.27 2e-138 hypothetical protein
blastp_kegg lcl|cit:102618810 1 255 + 255 Gaps:1 100.00 256 72.27 2e-138 acid phosphatase 1-like
blastp_kegg lcl|gmx:100785781 1 255 + 255 Gaps:21 100.00 276 65.94 2e-131 acid phosphatase 1-like
blastp_kegg lcl|pvu:PHAVU_001G164000g 1 255 + 255 Gaps:18 100.00 273 66.67 3e-131 hypothetical protein
blastp_kegg lcl|vvi:100246316 1 255 + 255 Gaps:1 100.00 256 70.31 2e-130 acid phosphatase 1-like
blastp_pdb 2i34_B 71 237 + 167 Gaps:8 62.40 258 26.09 9e-06 mol:protein length:258 acid phosphatase
blastp_pdb 2i34_A 71 237 + 167 Gaps:8 62.40 258 26.09 9e-06 mol:protein length:258 acid phosphatase
blastp_pdb 2i33_B 71 237 + 167 Gaps:8 62.40 258 26.09 9e-06 mol:protein length:258 Acid phosphatase
blastp_pdb 2i33_A 71 237 + 167 Gaps:8 62.40 258 26.09 9e-06 mol:protein length:258 Acid phosphatase
blastp_uniprot_sprot sp|P27061|PPA1_SOLLC 42 254 + 213 Gaps:1 83.14 255 41.98 9e-59 Acid phosphatase 1 OS Solanum lycopersicum GN APS1 PE 2 SV 1
blastp_uniprot_sprot sp|P15490|VSPA_SOYBN 3 254 + 252 Gaps:6 99.21 254 37.70 4e-55 Stem 28 kDa glycoprotein OS Glycine max GN VSPA PE 2 SV 1
blastp_uniprot_sprot sp|P10742|S25K_SOYBN 3 250 + 248 Gaps:8 84.54 291 38.62 2e-52 Stem 31 kDa glycoprotein (Fragment) OS Glycine max GN VSP25 PE 2 SV 2
blastp_uniprot_sprot sp|P10743|VSPB_SOYBN 42 254 + 213 Gaps:4 83.07 254 39.81 1e-48 Stem 31 kDa glycoprotein OS Glycine max GN VSPB PE 2 SV 1
blastp_uniprot_sprot sp|O49195|VSP1_ARATH 30 255 + 226 Gaps:8 82.96 270 38.39 8e-43 Vegetative storage protein 1 OS Arabidopsis thaliana GN VSP1 PE 1 SV 2
blastp_uniprot_sprot sp|O82122|VSP2_ARATH 42 255 + 214 Gaps:7 80.38 265 38.03 6e-42 Vegetative storage protein 2 OS Arabidopsis thaliana GN VSP2 PE 1 SV 1
blastp_uniprot_sprot sp|O04195|Y2992_ARATH 41 252 + 212 Gaps:30 66.43 283 27.13 8e-10 Uncharacterized protein At2g39920 OS Arabidopsis thaliana GN At2g39920 PE 2 SV 2
rpsblast_cdd gnl|CDD|130736 33 255 + 223 Gaps:1 96.94 229 53.15 2e-83 TIGR01675 plant-AP plant acid phosphatase. This model represents a family of acid phosphatase from plants which are most closely related to the (so called) class B non-specific acid phosphatase OlpA (TIGR01533 which is believed to be a 5'-nucleotide phosphatase) and somewhat more distantly to another class B phosphatase AphA (TIGR01672). Together these three clades define a subfamily (pfam03767) which corresponds to the IIIB subfamily of the haloacid dehalogenase (HAD) superfamily of aspartate nucleophile hydrolases. It has been reported that the best substrate for this enzyme that could be found was purine 5'-nucleoside phosphates. This is in concordance with the assignment of the H. influenzae hel protein (from TIGR01533) as a 5'-nucleotidase however there is presently no other evidence to support this specific function for these plant phosphatases. Many genes from this family have been annotated as vegetative storage proteins due to their close homology with these earlier-characterized gene products which are highly expressed in leaves. There are significant differences however including expression levels and distribution. The most important difference is the lack in authentic VSPs of the nucleophilic aspartate residue which is instead replaced by serine glycine or asparagine. Thus these proteins can not be expected to be active phosphatases. This issue was confused by the publication in 1992 of an article claiming activity for the Glycine max VSP. In 1994 this assertion was refuted by the separation of the activity from the VSP. This model explicitly excludes the VSPs which lack the nucleophilc aspartate. The possibility exists however that some members of this family may while containing all of the conserved HAD-superfamily catalytic residues lack activity and have a function related to the function of the VSPs rather than the acid phosphatases.
rpsblast_cdd gnl|CDD|202762 43 253 + 211 Gaps:2 99.06 213 45.50 1e-69 pfam03767 Acid_phosphat_B HAD superfamily subfamily IIIB (Acid phosphatase). This family proteins includes acid phosphatases and a number of vegetative storage proteins.
rpsblast_cdd gnl|CDD|130741 42 249 + 208 Gaps:6 75.64 275 41.35 1e-44 TIGR01680 Veg_Stor_Prot vegetative storage protein. The proteins represented by this model are close relatives of the plant acid phosphatases (TIGR01675) are limited to members of the Phaseoleae including Glycine max (soybean) and Phaseolus vulgaris (kidney bean). These proteins are highly expressed in the leaves of repeatedly depodded plants. VSP differs most strinkingly from the acid phosphatases in the lack of the conserved nucleophilic aspartate residue in the N-terminus thus they should be inactive as phosphatases. This issue was confused by the publication in 1992 of an article claiming activity for the Glycine max VSP. In 1994 this assertion was refuted by the separation of the activity from the VSP.
rpsblast_cdd gnl|CDD|162404 71 235 + 165 Gaps:8 59.77 266 25.16 3e-12 TIGR01533 lipo_e_P4 5'-nucleotidase lipoprotein e(P4) family. This model represents a set of bacterial lipoproteins belonging to a larger acid phosphatase family (pfam03767) which in turn belongs to the haloacid dehalogenase (HAD) superfamily of aspartate-dependent hydrolases. Members are found on the outer membrane of Gram-negative bacteria and the cytoplasmic membrane of Gram-positive bacteria. Most members have classic lipoprotein signal sequences. A critical role of this 5'-nucleotidase in Haemophilus influenzae is the degradation of external riboside in order to allow transport into the cell. An earlier suggested role in hemin transport is no longer current. This enzyme may also have other physiologically significant roles.
rpsblast_cdd gnl|CDD|32577 71 242 + 172 Gaps:9 60.95 274 25.15 4e-09 COG2503 COG2503 Predicted secreted acid phosphatase [General function prediction only].

12 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
TIGRFAM 35 254 220 TIGR01675 none plant-AP: plant acid phosphatase IPR010028
PIRSF 4 255 252 PIRSF002674 none none IPR014403
Phobius 1 21 21 SIGNAL_PEPTIDE none Signal peptide region none
Gene3D 71 237 167 G3DSA:3.40.50.1000 none none IPR023214
Pfam 38 254 217 PF03767 none HAD superfamily, subfamily IIIB (Acid phosphatase) IPR005519
PANTHER 36 253 218 PTHR31284:SF6 none none none
PANTHER 36 253 218 PTHR31284 none none none
SUPERFAMILY 97 241 145 SSF56784 none none IPR023214
Phobius 1 3 3 SIGNAL_PEPTIDE_N_REGION none N-terminal region of a signal peptide. none
Phobius 17 21 5 SIGNAL_PEPTIDE_C_REGION none C-terminal region of a signal peptide. none
Phobius 4 16 13 SIGNAL_PEPTIDE_H_REGION none Hydrophobic region of a signal peptide. none
Phobius 22 255 234 NON_CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. none

2 Localization

Analysis Start End Length
TMHMM 4 26 22
SignalP_EUK 1 21 20

2 Qtllist

Qtl Name Chromosome Name Linkage Group Prox Marker Dist Marker Position QTL Pos One Pos Two Test Type Test Value R 2
PM_1999_QTL15_peak_Bud_burst_3P Qrob_Chr09 9 s_1CGP2H_273 v_15801_330 27,16 9,16 47,16 lod 3,6 6,5
Bourran1_2003_QTL5_peak_Bud_burst_3P Qrob_Chr09 9 s_1ATM17_504 s_1AYZFM_899 29,81 19,81 41,81 lod 3,3 8,9

1 Targeting

Analysis Start End Length Location Reliability Signal Peptide Cut Off Mitochondrion Cut Off Network Signal Peptide Length
TargetP 1 21   Secretory pathway 1 0.917 0.040 NON-PLANT 21