Protein : Qrob_P0123210.2 Q. robur
Protein Identifier  ? Qrob_P0123210.2 Organism . Name  Quercus robur
Score  100.0 Score Type  egn
Protein Description  (M=2) K01893 - asparaginyl-tRNA synthetase [EC:6.1.1.22] Code Enzyme  EC:6.1.1.22
Gene Prediction Quality  validated Protein length 

Sequence

Length: 223  
Kegg Orthology  K01893
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0 Synonyms

5 GO Terms

Identifier Name Description
GO:0003676 nucleic acid binding Interacting selectively and non-covalently with any nucleic acid.
GO:0005524 ATP binding Interacting selectively and non-covalently with ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
GO:0000166 nucleotide binding Interacting selectively and non-covalently with a nucleotide, any compound consisting of a nucleoside that is esterified with (ortho)phosphate or an oligophosphate at any hydroxyl group on the ribose or deoxyribose.
GO:0004812 aminoacyl-tRNA ligase activity Catalysis of the formation of aminoacyl-tRNA from ATP, amino acid, and tRNA with the release of diphosphate and AMP.
GO:0006418 tRNA aminoacylation for protein translation The synthesis of aminoacyl tRNA by the formation of an ester bond between the 3'-hydroxyl group of the most 3' adenosine of the tRNA, to be used in ribosome-mediated polypeptide synthesis.

31 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|mdm:103432081 1 214 + 214 Gaps:14 40.95 569 75.11 5e-80 asparagine--tRNA ligase cytoplasmic 1-like
blastp_kegg lcl|cmo:103492241 1 214 + 214 Gaps:2 36.71 572 79.52 6e-80 asparagine--tRNA ligase cytoplasmic 1-like
blastp_kegg lcl|pxb:103936501 1 214 + 214 Gaps:14 40.95 569 74.68 1e-78 asparagine--tRNA ligase cytoplasmic 1-like
blastp_kegg lcl|mdm:103430180 1 168 + 168 Gaps:5 75.81 215 76.07 2e-77 asparagine--tRNA ligase cytoplasmic 1-like
blastp_kegg lcl|csv:101225750 1 214 + 214 Gaps:2 36.71 572 78.10 4e-77 asparagine--tRNA ligase cytoplasmic 1-like
blastp_kegg lcl|csv:101217798 1 214 + 214 Gaps:2 36.71 572 78.10 4e-77 asparagine--tRNA ligase cytoplasmic 1-like
blastp_kegg lcl|pxb:103965375 1 214 + 214 Gaps:14 40.95 569 72.96 1e-76 asparagine--tRNA ligase cytoplasmic 1-like
blastp_kegg lcl|pvu:PHAVU_002G305800g 4 214 + 211 Gaps:17 40.32 568 73.36 5e-76 hypothetical protein
blastp_kegg lcl|gmx:100804064 1 214 + 214 Gaps:17 40.56 567 73.04 1e-75 asparagine--tRNA ligase cytoplasmic 1-like
blastp_kegg lcl|gmx:100790605 1 214 + 214 Gaps:17 40.56 567 73.91 1e-75 asparagine--tRNA ligase cytoplasmic 1-like
blastp_uniprot_sprot sp|Q9SW96|SYNC1_ARATH 1 214 + 214 Gaps:15 41.26 572 62.29 8e-61 Asparagine--tRNA ligase cytoplasmic 1 OS Arabidopsis thaliana GN SYNC1 PE 2 SV 1
blastp_uniprot_sprot sp|Q9SSK1|SYNC3_ARATH 10 214 + 205 Gaps:19 39.40 571 64.44 9e-57 Asparagine--tRNA ligase cytoplasmic 3 OS Arabidopsis thaliana GN SYNC3 PE 3 SV 1
blastp_uniprot_sprot sp|O48593|SYNO_ARATH 31 214 + 184 Gaps:18 36.86 567 44.98 2e-22 Asparagine--tRNA ligase chloroplastic/mitochondrial OS Arabidopsis thaliana GN SYNO PE 2 SV 3
blastp_uniprot_sprot sp|B2RK42|SYN_PORG3 50 214 + 165 Gaps:10 43.71 469 41.95 3e-21 Asparagine--tRNA ligase OS Porphyromonas gingivalis (strain ATCC 33277 / DSM 20709 / JCM 12257) GN asnS PE 3 SV 1
blastp_uniprot_sprot sp|Q7MVE5|SYN_PORGI 50 214 + 165 Gaps:10 43.71 469 41.95 3e-21 Asparagine--tRNA ligase OS Porphyromonas gingivalis (strain ATCC BAA-308 / W83) GN asnS PE 3 SV 1
blastp_uniprot_sprot sp|A0LL53|SYN_SYNFM 54 213 + 160 Gaps:10 43.35 466 41.58 4e-21 Asparagine--tRNA ligase OS Syntrophobacter fumaroxidans (strain DSM 10017 / MPOB) GN asnS PE 3 SV 1
blastp_uniprot_sprot sp|Q023P9|SYN_SOLUE 39 214 + 176 Gaps:11 36.23 461 53.89 2e-20 Asparagine--tRNA ligase OS Solibacter usitatus (strain Ellin6076) GN asnS PE 3 SV 1
blastp_uniprot_sprot sp|Q6ANL4|SYN_DESPS 55 219 + 165 Gaps:14 46.77 449 44.29 4e-20 Asparagine--tRNA ligase OS Desulfotalea psychrophila (strain LSv54 / DSM 12343) GN asnS PE 3 SV 1
blastp_uniprot_sprot sp|P58692|SYN_NOSS1 50 214 + 165 Gaps:6 35.21 463 50.31 4e-20 Asparagine--tRNA ligase OS Nostoc sp. (strain PCC 7120 / UTEX 2576) GN asnS PE 3 SV 1
blastp_uniprot_sprot sp|Q1MQG9|SYN_LAWIP 57 214 + 158 Gaps:14 43.52 455 44.44 7e-20 Asparagine--tRNA ligase OS Lawsonia intracellularis (strain PHE/MN1-00) GN asnS PE 3 SV 1
rpsblast_cdd gnl|CDD|177867 1 214 + 214 Gaps:6 36.71 572 71.43 4e-65 PLN02221 PLN02221 asparaginyl-tRNA synthetase.
rpsblast_cdd gnl|CDD|179678 36 214 + 179 Gaps:11 37.78 450 48.82 4e-26 PRK03932 asnC asparaginyl-tRNA synthetase Validated.
rpsblast_cdd gnl|CDD|178147 75 214 + 140 Gaps:1 22.75 633 54.86 2e-24 PLN02532 PLN02532 asparagine-tRNA synthetase.
rpsblast_cdd gnl|CDD|200017 40 214 + 175 Gaps:7 38.41 453 47.70 1e-23 TIGR00457 asnS asparaginyl-tRNA synthetase. In a multiple sequence alignment of representative asparaginyl-tRNA synthetases (asnS) archaeal/eukaryotic type aspartyl-tRNA synthetases (aspS_arch) and bacterial type aspartyl-tRNA synthetases (aspS_bact) there is a striking similarity between asnS and aspS_arch in gap pattern and in sequence and a striking divergence of aspS_bact. Consequently a separate model was built for each of the three groups. This model asnS represents asparaginyl-tRNA synthetases from the three domains of life. Some species lack this enzyme and charge tRNA(asn) by misacylation with Asp followed by transamidation of Asp to Asn.
rpsblast_cdd gnl|CDD|178213 5 214 + 210 Gaps:12 36.46 565 43.20 1e-22 PLN02603 PLN02603 asparaginyl-tRNA synthetase.
rpsblast_cdd gnl|CDD|58588 57 138 + 82 Gaps:5 98.78 82 43.21 4e-18 cd04318 EcAsnRS_like_N EcAsnRS_like_N: N-terminal anticodon recognition domain of the type found in Escherichia coli asparaginyl-tRNA synthetase (AsnRS) and in Arabidopsis thaliana and Saccharomyces cerevisiae mitochondrial (mt) AsnRS. This domain is a beta-barrel domain (OB fold) involved in binding the tRNA anticodon stem-loop. The enzymes in this group are homodimeric class2b aminoacyl-tRNA synthetases (aaRSs). aaRSs catalyze the specific attachment of amino acids (AAs) to their cognate tRNAs during protein biosynthesis. This 2-step reaction involves i) the activation of the AA by ATP in the presence of magnesium ions followed by ii) the transfer of the activated AA to the terminal ribose of tRNA. In the case of the class2b aaRSs the activated AA is attached to the 3'OH of the terminal ribose. Eukaryotes contain 2 sets of aaRSs both of which are encoded by the nuclear genome. One set concerns with cytoplasmic protein synthesis whereas the other exclusively with mitochondrial protein synthesis. S. cerevisiae mtAsnRS can charge E.coli tRNA with asparagines. Mutations in the gene for S. cerevisiae mtAsnRS has been found to induce a "petite" phenotype typical for a mutation in a nuclear gene that results in a non-functioning mitochondrial protein synthesis system..
rpsblast_cdd gnl|CDD|185610 54 214 + 161 Gaps:15 29.35 586 47.09 6e-17 PTZ00425 PTZ00425 asparagine-tRNA ligase Provisional.
rpsblast_cdd gnl|CDD|30367 54 213 + 160 Gaps:12 36.55 435 40.88 1e-15 COG0017 AsnS Aspartyl/asparaginyl-tRNA synthetases [Translation ribosomal structure and biogenesis].
rpsblast_cdd gnl|CDD|29821 169 213 + 45 Gaps:1 14.29 322 47.83 1e-09 cd00776 AsxRS_core Asx tRNA synthetase (AspRS/AsnRS) class II core domain. Assignment to class II aminoacyl-tRNA synthetases (aaRS) based upon its structure and the presence of three characteristic sequence motifs in the core domain. This family includes AsnRS as well as a subgroup of AspRS. AsnRS and AspRS are homodimers which attach either asparagine or aspartate to the 3'OH group of ribose of the appropriate tRNA. While archaea lack asnRS they possess a non-discriminating aspRS which can mischarge Asp-tRNA with Asn. Subsequently a tRNA-dependent aspartate amidotransferase converts the bound aspartate to asparagine. The catalytic core domain is primarily responsible for the ATP-dependent formation of the enzyme bound aminoacyl-adenylate..
rpsblast_cdd gnl|CDD|58585 56 138 + 83 Gaps:10 100.00 85 28.24 2e-07 cd04100 Asp_Lys_Asn_RS_N Asp_Lys_Asn_RS_N: N-terminal anticodon recognition domain of class 2b aminoacyl-tRNA synthetases (aaRSs). This domain is a beta-barrel domain (OB fold) involved in binding the tRNA anticodon stem-loop. Class 2b aaRSs include the homodimeric aspartyl- asparaginyl- and lysyl-tRNA synthetases (AspRS AsnRS and LysRS). aaRSs catalyze the specific attachment of amino acids (AAs) to their cognate tRNAs during protein biosynthesis. This 2-step reaction involves i) the activation of the AA by ATP in the presence of magnesium ions followed by ii) the transfer of the activated AA to the terminal ribose of tRNA. In the case of the class2b aaRSs the activated AA is attached to the 3'OH of the terminal ribose. Eukaryotes contain 2 sets of aaRSs both of which are encoded by the nuclear genome. One set concerns with cytoplasmic protein synthesis whereas the other exclusively with mitochondrial protein synthesis. Included in this group are archeal and archeal-like AspRSs which are non-discriminating and can charge both tRNAAsp and tRNAAsn. E. coli cells have two isoforms of LysRSs (LysS and LysU) encoded by two distinct genes which are differentially regulated. The cytoplasmic and the mitochondrial isoforms of human LysRS are encoded by a single gene. Yeast cytoplasmic and mitochondrial LysRSs participate in mitochondrial import of cytoplasmic tRNAlysCUU. In addition to their housekeeping role human LysRS may function as a signaling molecule that activates immune cells. Tomato LysRS may participate in a process possibly connected to conditions of oxidative-stress conditions or heavy metal uptake. It is known that human tRNAlys and LysRS are specifically packaged into HIV-1 suggesting a role for LysRS in tRNA packaging. AsnRS is immunodominant antigen of the filarial nematode Brugia malayai and is of interest as a target for anti-parasitic drug design. Human AsnRS has been shown to be a pro-inflammatory chemokine which interacts with CCR3 chemokine receptors on T cells immature dendritic cells and macrophages..

8 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
Gene3D 53 140 88 G3DSA:2.40.50.140 none none IPR012340
PANTHER 39 168 130 PTHR22594 "Reactome:REACT_71";signature_desc=ASPARTYL/LYSYL-TRNA SYNTHETASE none IPR018150
SUPERFAMILY 169 214 46 SSF55681 none none none
Pfam 57 133 77 PF01336 none OB-fold nucleic acid binding domain IPR004365
SUPERFAMILY 50 137 88 SSF50249 none none IPR012340
Gene3D 166 214 49 G3DSA:3.30.930.10 none none none
Pfam 143 213 71 PF00152 "Reactome:REACT_71" tRNA synthetases class II (D, K and N) IPR004364
PANTHER 39 168 130 PTHR22594:SF28 none none none

0 Localization

5 Qtllist

Qtl Name Chromosome Name Linkage Group Prox Marker Dist Marker Position QTL Pos One Pos Two Test Type Test Value R 2
Bourran1_2003_QTL2_peak_Bud_burst_A4 Qrob_Chr02 2 s_1B0H8U_259 s_1CB1VL_554 17 0 87 lod 3,3 8,7
Bourran2_2004_QTL9_peak_Bud_burst_3P Qrob_Chr02 2 s_1C34E9_788 v_12238_322 50 25 75 lod 4,4 10,1
NancyGreenhouseCO2_2001_ambient_elevated_leaf_cellulose_QTL2_d13Cf Qrob_Chr02 2 s_1AQA4Z_1644 s_1AK5QX_947 53.67 14,01 79,68 lod 5.6594 0.03
Bourran2_2003_QTL8_peak_Bud_burst_3P Qrob_Chr02 2 s_1ANG6_1446 v_11270_161 40 0 72 lod 4,4 9,9
Bourran2_2014_nP_A4 Qrob_Chr11 11 s_1B58GB_1413 s_1A5BYY_1671 11,15 0 42,38 lod 1,8913 4,5

0 Targeting