Protein : Qrob_P0110280.2 Q. robur
Protein Identifier  ? Qrob_P0110280.2 Organism . Name  Quercus robur
Score  100.0 Score Type  egn
Protein Description  (M=25) 3.1.3.2 - Acid phosphatase. Code Enzyme  EC:3.1.3.2
Gene Prediction Quality  validated Protein length 

Sequence

Length: 247  
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0 Synonyms

1 GO Terms

Identifier Name Description
GO:0003993 acid phosphatase activity Catalysis of the reaction: an orthophosphoric monoester + H2O = an alcohol + phosphate, with an acid pH optimum.

26 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|pmum:103327818 1 246 + 246 Gaps:23 98.49 265 53.64 3e-87 acid phosphatase 1-like
blastp_kegg lcl|sot:102598975 1 246 + 246 Gaps:24 100.00 264 49.24 3e-86 acid phosphatase 1-like
blastp_kegg lcl|pper:PRUPE_ppa010152mg 1 246 + 246 Gaps:23 100.00 261 52.49 5e-86 hypothetical protein
blastp_kegg lcl|fve:101300364 1 246 + 246 Gaps:25 99.25 265 52.09 9e-86 stem 28 kDa glycoprotein-like
blastp_kegg lcl|pop:POPTR_0001s19180g 8 246 + 239 Gaps:22 96.93 261 53.36 2e-85 POPTRDRAFT_705836 hypothetical protein
blastp_kegg lcl|rcu:RCOM_1487980 7 246 + 240 Gaps:21 98.06 258 52.57 1e-84 Stem 28 kDa glycoprotein precursor putative (EC:3.1.3.2)
blastp_kegg lcl|sly:101263824 19 245 + 227 Gaps:3 84.21 266 57.14 8e-84 acid phosphatase 1-like
blastp_kegg lcl|tcc:TCM_028914 3 246 + 244 Gaps:22 99.61 259 51.55 2e-83 Stem 28 kDa glycoprotein putative
blastp_kegg lcl|cit:102626130 1 246 + 246 Gaps:24 99.24 264 51.53 7e-81 acid phosphatase 1-like
blastp_kegg lcl|sly:101264133 1 245 + 245 Gaps:25 94.96 278 46.21 7e-81 acid phosphatase 1-like
blastp_pdb 2i34_B 95 243 + 149 Gaps:30 60.85 258 31.85 5e-10 mol:protein length:258 acid phosphatase
blastp_pdb 2i34_A 95 243 + 149 Gaps:30 60.85 258 31.85 5e-10 mol:protein length:258 acid phosphatase
blastp_pdb 2i33_B 95 243 + 149 Gaps:30 60.85 258 31.85 5e-10 mol:protein length:258 Acid phosphatase
blastp_pdb 2i33_A 95 243 + 149 Gaps:30 60.85 258 31.85 5e-10 mol:protein length:258 Acid phosphatase
blastp_uniprot_sprot sp|P27061|PPA1_SOLLC 30 245 + 216 Gaps:4 83.14 255 47.64 1e-66 Acid phosphatase 1 OS Solanum lycopersicum GN APS1 PE 2 SV 1
blastp_uniprot_sprot sp|P15490|VSPA_SOYBN 1 245 + 245 Gaps:22 99.61 254 44.66 1e-58 Stem 28 kDa glycoprotein OS Glycine max GN VSPA PE 2 SV 1
blastp_uniprot_sprot sp|P10742|S25K_SOYBN 6 241 + 236 Gaps:22 83.85 291 44.26 3e-54 Stem 31 kDa glycoprotein (Fragment) OS Glycine max GN VSP25 PE 2 SV 2
blastp_uniprot_sprot sp|P10743|VSPB_SOYBN 30 246 + 217 Gaps:9 83.46 254 47.64 3e-54 Stem 31 kDa glycoprotein OS Glycine max GN VSPB PE 2 SV 1
blastp_uniprot_sprot sp|O49195|VSP1_ARATH 29 245 + 217 Gaps:12 78.89 270 45.07 1e-47 Vegetative storage protein 1 OS Arabidopsis thaliana GN VSP1 PE 1 SV 2
blastp_uniprot_sprot sp|O82122|VSP2_ARATH 29 245 + 217 Gaps:8 80.38 265 41.78 3e-43 Vegetative storage protein 2 OS Arabidopsis thaliana GN VSP2 PE 1 SV 1
blastp_uniprot_sprot sp|O04195|Y2992_ARATH 3 243 + 241 Gaps:40 78.09 283 25.79 2e-09 Uncharacterized protein At2g39920 OS Arabidopsis thaliana GN At2g39920 PE 2 SV 2
rpsblast_cdd gnl|CDD|130736 30 245 + 216 Gaps:4 92.58 229 50.94 1e-73 TIGR01675 plant-AP plant acid phosphatase. This model represents a family of acid phosphatase from plants which are most closely related to the (so called) class B non-specific acid phosphatase OlpA (TIGR01533 which is believed to be a 5'-nucleotide phosphatase) and somewhat more distantly to another class B phosphatase AphA (TIGR01672). Together these three clades define a subfamily (pfam03767) which corresponds to the IIIB subfamily of the haloacid dehalogenase (HAD) superfamily of aspartate nucleophile hydrolases. It has been reported that the best substrate for this enzyme that could be found was purine 5'-nucleoside phosphates. This is in concordance with the assignment of the H. influenzae hel protein (from TIGR01533) as a 5'-nucleotidase however there is presently no other evidence to support this specific function for these plant phosphatases. Many genes from this family have been annotated as vegetative storage proteins due to their close homology with these earlier-characterized gene products which are highly expressed in leaves. There are significant differences however including expression levels and distribution. The most important difference is the lack in authentic VSPs of the nucleophilic aspartate residue which is instead replaced by serine glycine or asparagine. Thus these proteins can not be expected to be active phosphatases. This issue was confused by the publication in 1992 of an article claiming activity for the Glycine max VSP. In 1994 this assertion was refuted by the separation of the activity from the VSP. This model explicitly excludes the VSPs which lack the nucleophilc aspartate. The possibility exists however that some members of this family may while containing all of the conserved HAD-superfamily catalytic residues lack activity and have a function related to the function of the VSPs rather than the acid phosphatases.
rpsblast_cdd gnl|CDD|202762 31 245 + 215 Gaps:5 99.53 213 54.25 1e-72 pfam03767 Acid_phosphat_B HAD superfamily subfamily IIIB (Acid phosphatase). This family proteins includes acid phosphatases and a number of vegetative storage proteins.
rpsblast_cdd gnl|CDD|130741 6 246 + 241 Gaps:31 92.36 275 42.13 2e-49 TIGR01680 Veg_Stor_Prot vegetative storage protein. The proteins represented by this model are close relatives of the plant acid phosphatases (TIGR01675) are limited to members of the Phaseoleae including Glycine max (soybean) and Phaseolus vulgaris (kidney bean). These proteins are highly expressed in the leaves of repeatedly depodded plants. VSP differs most strinkingly from the acid phosphatases in the lack of the conserved nucleophilic aspartate residue in the N-terminus thus they should be inactive as phosphatases. This issue was confused by the publication in 1992 of an article claiming activity for the Glycine max VSP. In 1994 this assertion was refuted by the separation of the activity from the VSP.
rpsblast_cdd gnl|CDD|162404 63 243 + 181 Gaps:38 73.31 266 31.28 3e-14 TIGR01533 lipo_e_P4 5'-nucleotidase lipoprotein e(P4) family. This model represents a set of bacterial lipoproteins belonging to a larger acid phosphatase family (pfam03767) which in turn belongs to the haloacid dehalogenase (HAD) superfamily of aspartate-dependent hydrolases. Members are found on the outer membrane of Gram-negative bacteria and the cytoplasmic membrane of Gram-positive bacteria. Most members have classic lipoprotein signal sequences. A critical role of this 5'-nucleotidase in Haemophilus influenzae is the degradation of external riboside in order to allow transport into the cell. An earlier suggested role in hemin transport is no longer current. This enzyme may also have other physiologically significant roles.
rpsblast_cdd gnl|CDD|32577 31 243 + 213 Gaps:38 82.85 274 26.87 3e-11 COG2503 COG2503 Predicted secreted acid phosphatase [General function prediction only].

11 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
PIRSF 8 246 239 PIRSF002674 none none IPR014403
PANTHER 25 245 221 PTHR31284 none none none
Phobius 3 14 12 SIGNAL_PEPTIDE_H_REGION none Hydrophobic region of a signal peptide. none
Phobius 1 19 19 SIGNAL_PEPTIDE none Signal peptide region none
Phobius 15 19 5 SIGNAL_PEPTIDE_C_REGION none C-terminal region of a signal peptide. none
Phobius 1 2 2 SIGNAL_PEPTIDE_N_REGION none N-terminal region of a signal peptide. none
SUPERFAMILY 92 243 152 SSF56784 none none IPR023214
Gene3D 94 234 141 G3DSA:3.40.50.1000 none none IPR023214
Phobius 20 246 227 NON_CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. none
TIGRFAM 28 245 218 TIGR01675 none plant-AP: plant acid phosphatase IPR010028
Pfam 26 245 220 PF03767 none HAD superfamily, subfamily IIIB (Acid phosphatase) IPR005519

3 Localization

Analysis Start End Length
SignalP_GRAM_POSITIVE 1 20 19
SignalP_GRAM_NEGATIVE 1 19 18
SignalP_EUK 1 19 18

2 Qtllist

Qtl Name Chromosome Name Linkage Group Prox Marker Dist Marker Position QTL Pos One Pos Two Test Type Test Value R 2
Bourran2_2014_nSecLBD_3P Qrob_Chr08 8 s_1BN2OD_551 s_1B5AYF_599 17,17 0 43,51 lod 1,9229 4,4
Bourran2_2014_rEpiBC_3P Qrob_Chr05 5 s_2GDU0O_517 s_1BT8O3_239 25,81 0 55,45 lod 2,2101 5,5

1 Targeting

Analysis Start End Length Location Reliability Signal Peptide Cut Off Mitochondrion Cut Off Network Signal Peptide Length
TargetP 1 19   Secretory pathway 1 0.938 0.054 NON-PLANT 19