Protein : Qrob_P0098500.2 Q. robur
Protein Identifier  ? Qrob_P0098500.2 Organism . Name  Quercus robur
Score  100.0 Score Type  egn
Protein Description  (M=34) 1.14.13.89 - Isoflavone 2'-hydroxylase. Code Enzyme  EC:1.14.13.89
Gene Prediction Quality  validated Protein length 

Sequence

Length: 507  
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0 Synonyms

4 GO Terms

Identifier Name Description
GO:0005506 iron ion binding Interacting selectively and non-covalently with iron (Fe) ions.
GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen Catalysis of an oxidation-reduction (redox) reaction in which hydrogen or electrons are transferred from each of two donors, and molecular oxygen is reduced or incorporated into a donor.
GO:0020037 heme binding Interacting selectively and non-covalently with heme, any compound of iron complexed in a porphyrin (tetrapyrrole) ring.
GO:0055114 oxidation-reduction process A metabolic process that results in the removal or addition of one or more electrons to or from a substance, with or without the concomitant removal or addition of a proton or protons.

45 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|pper:PRUPE_ppa019965mg 1 500 + 500 Gaps:3 100.00 499 68.54 0.0 hypothetical protein
blastp_kegg lcl|pmum:103337275 1 500 + 500 Gaps:3 100.00 499 67.94 0.0 isoflavone 2'-hydroxylase-like
blastp_kegg lcl|fve:101304511 1 500 + 500 Gaps:3 100.00 499 67.94 0.0 isoflavone 2'-hydroxylase-like
blastp_kegg lcl|pper:PRUPE_ppa004635mg 1 500 + 500 Gaps:3 100.00 499 66.73 0.0 hypothetical protein
blastp_kegg lcl|rcu:RCOM_1154590 28 501 + 474 Gaps:6 94.63 503 67.02 0.0 cytochrome P450 putative (EC:1.14.13.89)
blastp_kegg lcl|pmum:103337298 1 500 + 500 Gaps:3 100.00 499 67.54 0.0 cytochrome P450 81D11-like
blastp_kegg lcl|vvi:100259358 1 506 + 506 Gaps:6 100.00 508 62.80 0.0 isoflavone 2'-hydroxylase-like
blastp_kegg lcl|pper:PRUPE_ppa023786mg 2 499 + 498 Gaps:4 99.01 503 63.65 0.0 hypothetical protein
blastp_kegg lcl|pmum:103337257 2 499 + 498 Gaps:4 99.01 503 63.86 0.0 isoflavone 2'-hydroxylase-like
blastp_kegg lcl|pmum:103337245 2 498 + 497 Gaps:8 99.40 496 65.52 0.0 cytochrome P450 81D11-like
blastp_pdb 2hi4_A 23 482 + 460 Gaps:50 95.35 495 26.91 1e-45 mol:protein length:495 Cytochrome P450 1A2
blastp_pdb 3swz_D 34 462 + 429 Gaps:40 88.87 494 28.25 1e-41 mol:protein length:494 Steroid 17-alpha-hydroxylase/17 20 lyase
blastp_pdb 3swz_C 34 462 + 429 Gaps:40 88.87 494 28.25 1e-41 mol:protein length:494 Steroid 17-alpha-hydroxylase/17 20 lyase
blastp_pdb 3swz_B 34 462 + 429 Gaps:40 88.87 494 28.25 1e-41 mol:protein length:494 Steroid 17-alpha-hydroxylase/17 20 lyase
blastp_pdb 3swz_A 34 462 + 429 Gaps:40 88.87 494 28.25 1e-41 mol:protein length:494 Steroid 17-alpha-hydroxylase/17 20 lyase
blastp_pdb 3ruk_D 34 462 + 429 Gaps:40 88.87 494 28.25 1e-41 mol:protein length:494 Steroid 17-alpha-hydroxylase/17 20 lyase
blastp_pdb 3ruk_C 34 462 + 429 Gaps:40 88.87 494 28.25 1e-41 mol:protein length:494 Steroid 17-alpha-hydroxylase/17 20 lyase
blastp_pdb 3ruk_B 34 462 + 429 Gaps:40 88.87 494 28.25 1e-41 mol:protein length:494 Steroid 17-alpha-hydroxylase/17 20 lyase
blastp_pdb 3ruk_A 34 462 + 429 Gaps:40 88.87 494 28.25 1e-41 mol:protein length:494 Steroid 17-alpha-hydroxylase/17 20 lyase
blastp_pdb 3qz1_D 32 459 + 428 Gaps:48 84.68 496 29.05 4e-40 mol:protein length:496 Steroid 21-hydroxylase
blastp_uniprot_sprot sp|P93147|C81E1_GLYEC 5 501 + 497 Gaps:5 99.00 499 56.88 0.0 Isoflavone 2'-hydroxylase OS Glycyrrhiza echinata GN CYP81E1 PE 1 SV 2
blastp_uniprot_sprot sp|Q9FG65|C81D1_ARATH 4 500 + 497 Gaps:16 98.61 502 58.38 0.0 Cytochrome P450 81D1 OS Arabidopsis thaliana GN CYP81D1 PE 2 SV 1
blastp_uniprot_sprot sp|O65790|C81F1_ARATH 5 499 + 495 Gaps:14 99.80 500 53.91 0.0 Cytochrome P450 81F1 OS Arabidopsis thaliana GN CYP81F1 PE 2 SV 2
blastp_uniprot_sprot sp|Q9SZ46|C82C4_ARATH 1 492 + 492 Gaps:29 98.66 524 40.04 1e-116 Cytochrome P450 82C4 OS Arabidopsis thaliana GN CYP82C4 PE 2 SV 1
blastp_uniprot_sprot sp|O49394|C82C2_ARATH 1 492 + 492 Gaps:28 98.66 523 38.57 2e-112 Cytochrome P450 82C2 OS Arabidopsis thaliana GN CYP82C2 PE 2 SV 2
blastp_uniprot_sprot sp|Q9LSF8|C82G1_ARATH 6 499 + 494 Gaps:32 98.64 515 38.39 5e-112 Cytochrome P450 82G1 OS Arabidopsis thaliana GN CYP82G1 PE 1 SV 1
blastp_uniprot_sprot sp|Q43068|C82A1_PEA 4 490 + 487 Gaps:49 97.79 544 37.03 6e-111 Cytochrome P450 82A1 (Fragment) OS Pisum sativum GN CYP82A1 PE 2 SV 2
blastp_uniprot_sprot sp|Q9SD85|F3PH_ARATH 6 492 + 487 Gaps:28 97.27 513 38.88 1e-109 Flavonoid 3'-monooxygenase OS Arabidopsis thaliana GN CYP75B1 PE 1 SV 1
blastp_uniprot_sprot sp|O49858|C82A3_SOYBN 16 492 + 477 Gaps:23 94.50 527 38.15 7e-109 Cytochrome P450 82A3 OS Glycine max GN CYP82A3 PE 2 SV 1
blastp_uniprot_sprot sp|O81972|C82A2_SOYBN 37 492 + 456 Gaps:25 91.38 522 38.99 8e-107 Cytochrome P450 82A2 OS Glycine max GN CYP82A2 PE 2 SV 1

25 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
PANTHER 1 501 501 PTHR24298 none none none
SUPERFAMILY 34 492 459 SSF48264 "Reactome:REACT_13433" none IPR001128
Gene3D 26 492 467 G3DSA:1.10.630.10 "Reactome:REACT_13433" none IPR001128
Phobius 14 18 5 SIGNAL_PEPTIDE_C_REGION none C-terminal region of a signal peptide. none
PRINTS 85 106 22 PR00463 none E-class P450 group I signature IPR002401
PRINTS 355 373 19 PR00463 none E-class P450 group I signature IPR002401
PRINTS 292 309 18 PR00463 none E-class P450 group I signature IPR002401
PRINTS 180 198 19 PR00463 none E-class P450 group I signature IPR002401
PRINTS 428 438 11 PR00463 none E-class P450 group I signature IPR002401
PRINTS 438 461 24 PR00463 none E-class P450 group I signature IPR002401
PRINTS 61 80 20 PR00463 none E-class P450 group I signature IPR002401
PRINTS 312 338 27 PR00463 none E-class P450 group I signature IPR002401
PRINTS 396 420 25 PR00463 none E-class P450 group I signature IPR002401
PRINTS 429 438 10 PR00385 "Reactome:REACT_13433" P450 superfamily signature IPR001128
PRINTS 303 320 18 PR00385 "Reactome:REACT_13433" P450 superfamily signature IPR001128
PRINTS 438 449 12 PR00385 "Reactome:REACT_13433" P450 superfamily signature IPR001128
PRINTS 356 367 12 PR00385 "Reactome:REACT_13433" P450 superfamily signature IPR001128
Coils 238 259 22 Coil none none none
Pfam 34 481 448 PF00067 "Reactome:REACT_13433" Cytochrome P450 IPR001128
Phobius 19 506 488 NON_CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. none
Phobius 1 18 18 SIGNAL_PEPTIDE none Signal peptide region none
Phobius 3 13 11 SIGNAL_PEPTIDE_H_REGION none Hydrophobic region of a signal peptide. none
ProSitePatterns 431 440 10 PS00086 "Reactome:REACT_13433" Cytochrome P450 cysteine heme-iron ligand signature. IPR017972
Phobius 1 2 2 SIGNAL_PEPTIDE_N_REGION none N-terminal region of a signal peptide. none
PANTHER 1 501 501 PTHR24298:SF61 none none none

0 Localization

13 Qtllist

Qtl Name Chromosome Name Linkage Group Prox Marker Dist Marker Position QTL Pos One Pos Two Test Type Test Value R 2
Bourran2_2014_nLBD*_A4 Qrob_Chr08 8 v_12498_318 v_12364_308 34,91 16,12 53,62 lod 2,4961 5,2
Bourran2_2014_nPriLBD_3P Qrob_Chr08 8 v_5216_549 v_11837_70 12,36 0 30,43 lod 2,5806 5,1
Bourran2_2014_nEpis*_A4 Qrob_Chr07 7 v_12400_446 s_1BPEBU_1211 6,93 0 15,13 lod 4,7411 11
Bourran2_2014_nSecLBD_A4 Qrob_Chr07 7 v_8327_222 s_1A4WGY_363 16,04 0 44,69 lod 2,6373 6,5
Bourran2_2014_nP_A4 Qrob_Chr11 11 s_1B58GB_1413 s_1A5BYY_1671 11,15 0 42,38 lod 1,8913 4,5
Bourran2_2014_nP_3P Qrob_Chr11 11 v_11486_194 s_1AT3E_2335 7,9 0,09 30,09 lod 2,3636 5
Bourran2_2015_nEpis_A4 Qrob_Chr09 9 v_15847_485 v_8329_369 34,94 34,88 37,45 lod 3.1 7
Bourran2_2015_nEpiBC_A4 Qrob_Chr07 7 s_1DP9TW_798 v_8128_173 22,61 22,14 22,73 lod 3.1 8.5
Bourran2_2014_nEpiBC_A4 Qrob_Chr07 7 s_2FI9D9_500 s_1AXDMJ_325 12,26 0 34,9 lod 2,2306 6,1
Bourran2_2014_nPriBD_3P Qrob_Chr11 11 v_11486_194 s_1AT3E_2335 5,54 0,4 20,6 lod 2,6345 5,9
Bourran2_2002_QTL10_peak_Bud_burst_3P Qrob_Chr07 7 s_1CAWP_311 s_1BEZ9M_461 7,5 0 21,5 lod 3,7 6,3
Bourran2_2014_nPriBD*_A4 Qrob_Chr07 7 s_1A7UI0_596 s_1A3H6S_352 8,02 0 20,53 lod 4,1062 10,8
Bourran2_2014_nPriLBD*_A4 Qrob_Chr07 7 s_1C6BY7_802 s_1BPEBU_1211 7,15 0 16,34 lod 3,9419 10,2

1 Targeting

Analysis Start End Length Location Reliability Signal Peptide Cut Off Mitochondrion Cut Off Network Signal Peptide Length
TargetP 1 30   Secretory pathway 1 0.933 0.054 NON-PLANT 30