Protein : Qrob_P0061440.2 Q. robur
Protein Identifier  ? Qrob_P0061440.2 Organism . Name  Quercus robur
Score  28.0 Score Type  egn
Protein Description  (M=4) 6.1.1.5 - Isoleucine--tRNA ligase. Code Enzyme  EC:6.1.1.5
Gene Prediction Quality  validated Protein length 

Sequence

Length: 534  
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0 Synonyms

4 GO Terms

Identifier Name Description
GO:0005524 ATP binding Interacting selectively and non-covalently with ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
GO:0000166 nucleotide binding Interacting selectively and non-covalently with a nucleotide, any compound consisting of a nucleoside that is esterified with (ortho)phosphate or an oligophosphate at any hydroxyl group on the ribose or deoxyribose.
GO:0004812 aminoacyl-tRNA ligase activity Catalysis of the formation of aminoacyl-tRNA from ATP, amino acid, and tRNA with the release of diphosphate and AMP.
GO:0006418 tRNA aminoacylation for protein translation The synthesis of aminoacyl tRNA by the formation of an ester bond between the 3'-hydroxyl group of the most 3' adenosine of the tRNA, to be used in ribosome-mediated polypeptide synthesis.

40 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|csv:101232219 1 130 + 130 none 84.97 153 83.08 4e-78 isoleucine--tRNA ligase-like
blastp_kegg lcl|pvu:PHAVU_011G075600g 1 127 + 127 none 31.75 400 82.68 3e-69 hypothetical protein
blastp_kegg lcl|csv:101212830 1 130 + 130 none 10.98 1184 83.08 3e-68 IARS isoleucyl-tRNA synthetase
blastp_kegg lcl|pop:POPTR_0007s02170g 1 127 + 127 none 10.77 1179 85.04 3e-68 POPTRDRAFT_216568 isoleucyl-tRNA synthetase family protein
blastp_kegg lcl|cmo:103494549 1 130 + 130 none 10.98 1184 83.08 4e-68 probable isoleucine--tRNA ligase cytoplasmic
blastp_kegg lcl|aly:ARALYDRAFT_489875 1 130 + 130 none 10.92 1190 80.77 2e-67 hypothetical protein
blastp_kegg lcl|crb:CARUB_v10000080mg 1 127 + 127 none 11.10 1144 81.89 2e-67 hypothetical protein
blastp_kegg lcl|rcu:RCOM_0574620 1 130 + 130 none 11.06 1175 82.31 2e-67 isoleucyl tRNA synthetase putative (EC:6.1.1.5)
blastp_kegg lcl|pop:POPTR_0017s06170g 1 130 + 130 none 11.27 1154 83.08 3e-67 hypothetical protein
blastp_kegg lcl|ath:AT4G10320 1 127 + 127 none 10.67 1190 81.89 9e-67 tRNA synthetase class I (I L M and V) family protein
blastp_pdb 1jzs_A 11 125 + 115 none 14.01 821 46.09 4e-30 mol:protein length:821 Isoleucyl-tRNA synthetase
blastp_pdb 1jzq_A 11 125 + 115 none 14.01 821 46.09 4e-30 mol:protein length:821 Isoleucyl-tRNA synthetase
blastp_pdb 1ile_A 11 125 + 115 none 14.01 821 46.09 4e-30 mol:protein length:821 ISOLEUCYL-TRNA SYNTHETASE
blastp_pdb 1qu3_A 13 125 + 113 Gaps:5 11.78 917 35.19 4e-16 mol:protein length:917 ISOLEUCYL-TRNA SYNTHETASE
blastp_pdb 1qu2_A 13 125 + 113 Gaps:5 11.78 917 35.19 4e-16 mol:protein length:917 ISOLEUCYL-TRNA SYNTHETASE
blastp_pdb 1ffy_A 13 125 + 113 Gaps:5 11.78 917 35.19 4e-16 mol:protein length:917 ISOLEUCYL-TRNA SYNTHETASE
blastp_uniprot_sprot sp|P36422|SYI_TETTH 11 129 + 119 none 11.01 1081 68.07 9e-50 Isoleucine--tRNA ligase OS Tetrahymena thermophila GN ILSA PE 3 SV 1
blastp_uniprot_sprot sp|P09436|SYIC_YEAST 10 125 + 116 none 10.82 1072 66.38 2e-46 Isoleucine--tRNA ligase cytoplasmic OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN ILS1 PE 1 SV 1
blastp_uniprot_sprot sp|P41252|SYIC_HUMAN 8 130 + 123 none 9.75 1262 60.98 1e-45 Isoleucine--tRNA ligase cytoplasmic OS Homo sapiens GN IARS PE 1 SV 2
blastp_uniprot_sprot sp|Q8BU30|SYIC_MOUSE 8 127 + 120 none 9.51 1262 62.50 3e-44 Isoleucine--tRNA ligase cytoplasmic OS Mus musculus GN Iars PE 2 SV 2
blastp_uniprot_sprot sp|Q54YD4|SYIC_DICDI 1 127 + 127 Gaps:2 11.72 1067 61.60 3e-44 Probable isoleucine--tRNA ligase cytoplasmic OS Dictyostelium discoideum GN ileS PE 3 SV 1
blastp_uniprot_sprot sp|O13651|SYIC_SCHPO 10 125 + 116 none 10.90 1064 62.07 5e-43 Isoleucine--tRNA ligase cytoplasmic OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN irs1 PE 3 SV 1
blastp_uniprot_sprot sp|B7XK16|SYIC_ENTBH 11 130 + 120 none 12.59 953 60.00 5e-42 Probable isoleucine--tRNA ligase cytoplasmic OS Enterocytozoon bieneusi (strain H348) GN EBI_22873 PE 3 SV 1
blastp_uniprot_sprot sp|Q6MDY1|SYI_PARUW 11 127 + 117 none 11.27 1038 59.83 2e-40 Isoleucine--tRNA ligase OS Protochlamydia amoebophila (strain UWE25) GN ileS PE 3 SV 1
blastp_uniprot_sprot sp|Q8SQV6|SYIC_ENCCU 8 127 + 120 none 11.80 1017 57.50 2e-39 Probable isoleucine--tRNA ligase cytoplasmic OS Encephalitozoon cuniculi (strain GB-M1) GN ECU11_1100 PE 3 SV 1
blastp_uniprot_sprot sp|Q9PL20|SYI_CHLMU 11 127 + 117 none 11.29 1036 57.26 4e-39 Isoleucine--tRNA ligase OS Chlamydia muridarum (strain MoPn / Nigg) GN ileS PE 3 SV 1
rpsblast_cdd gnl|CDD|178470 3 127 + 125 none 10.79 1159 84.00 2e-77 PLN02882 PLN02882 aminoacyl-tRNA ligase.
rpsblast_cdd gnl|CDD|180364 8 127 + 120 none 12.31 975 51.67 1e-52 PRK06039 ileS isoleucyl-tRNA synthetase Reviewed.
rpsblast_cdd gnl|CDD|143905 18 127 + 110 none 18.15 606 44.55 2e-42 pfam00133 tRNA-synt_1 tRNA synthetases class I (I L M and V). Other tRNA synthetase sub-families are too dissimilar to be included.
rpsblast_cdd gnl|CDD|173617 14 127 + 114 none 9.46 1205 58.77 4e-42 PTZ00427 PTZ00427 isoleucine-tRNA ligase putative Provisional.

17 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
Phobius 347 357 11 CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. none
Phobius 298 315 18 TRANSMEMBRANE none Region of a membrane-bound protein predicted to be embedded in the membrane. none
Phobius 258 278 21 TRANSMEMBRANE none Region of a membrane-bound protein predicted to be embedded in the membrane. none
PANTHER 8 127 120 PTHR11946:SF11 none none none
PANTHER 8 127 120 PTHR11946 none none none
Phobius 316 326 11 NON_CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. none
Phobius 279 297 19 CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. none
Phobius 423 533 111 CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. none
Phobius 383 401 19 NON_CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. none
Phobius 358 382 25 TRANSMEMBRANE none Region of a membrane-bound protein predicted to be embedded in the membrane. none
Gene3D 8 129 122 G3DSA:3.40.50.620 none none IPR014729
ProSitePatterns 49 60 12 PS00178 "Reactome:REACT_71" Aminoacyl-transfer RNA synthetases class-I signature. IPR001412
Phobius 1 257 257 NON_CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. none
Pfam 19 128 110 PF00133 "Reactome:REACT_71" tRNA synthetases class I (I, L, M and V) IPR002300
Phobius 402 422 21 TRANSMEMBRANE none Region of a membrane-bound protein predicted to be embedded in the membrane. none
Phobius 327 346 20 TRANSMEMBRANE none Region of a membrane-bound protein predicted to be embedded in the membrane. none
SUPERFAMILY 10 128 119 SSF52374 none none none

4 Localization

Analysis Start End Length
TMHMM 256 278 22
TMHMM 394 416 22
TMHMM 323 340 17
TMHMM 345 367 22

1 Qtllist

Qtl Name Chromosome Name Linkage Group Prox Marker Dist Marker Position QTL Pos One Pos Two Test Type Test Value R 2
Champenoux_2015_nPriLBD_3P Qrob_Chr10 10 v_15000_157 v_15000_310 15,68 15,9 15,91 lod 2.4 5.5

0 Targeting