Protein : Qrob_P0057170.2 Q. robur
Protein Identifier  ? Qrob_P0057170.2 Organism . Name  Quercus robur
Score  8.2 Score Type  egn
Protein Description  (M=6) PTHR10161 - TARTRATE-RESISTANT ACID PHOSPHATASE TYPE 5 Code Enzyme  EC:3.1.3.2
Gene Prediction Quality  validated Protein length 

Sequence

Length: 333  
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0 Synonyms

2 GO Terms

Identifier Name Description
GO:0003993 acid phosphatase activity Catalysis of the reaction: an orthophosphoric monoester + H2O = an alcohol + phosphate, with an acid pH optimum.
GO:0016787 hydrolase activity Catalysis of the hydrolysis of various bonds, e.g. C-O, C-N, C-C, phosphoric anhydride bonds, etc. Hydrolase is the systematic name for any enzyme of EC class 3.

30 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|pop:POPTR_0008s13850g 9 330 + 322 none 99.08 325 74.84 0.0 PURPLE ACID PHOSPHATASE PRECURSOR family protein
blastp_kegg lcl|pop:POPTR_0008s13870g 9 330 + 322 none 97.87 329 74.84 0.0 POPTRDRAFT_1085232 hypothetical protein
blastp_kegg lcl|vvi:100261163 9 328 + 320 none 98.77 324 75.31 0.0 purple acid phosphatase 3-like
blastp_kegg lcl|pmum:103320981 1 332 + 332 Gaps:4 88.19 381 72.02 2e-180 purple acid phosphatase 8-like
blastp_kegg lcl|pper:PRUPE_ppa008147mg 1 332 + 332 Gaps:4 97.96 343 72.02 2e-180 hypothetical protein
blastp_kegg lcl|pxb:103943113 13 330 + 318 Gaps:4 96.41 334 74.53 4e-180 purple acid phosphatase 3
blastp_kegg lcl|pper:PRUPE_ppa008436mg 7 329 + 323 Gaps:1 97.59 332 72.84 6e-177 hypothetical protein
blastp_kegg lcl|cic:CICLE_v10015905mg 9 330 + 322 Gaps:2 98.78 328 74.38 2e-176 hypothetical protein
blastp_kegg lcl|cit:102617818 9 330 + 322 Gaps:2 98.78 328 74.38 2e-176 purple acid phosphatase 8-like
blastp_kegg lcl|rcu:RCOM_1150470 7 332 + 326 Gaps:2 99.39 328 72.39 2e-176 Tartrate-resistant acid phosphatase type 5 precursor putative (EC:3.1.3.2)
blastp_pdb 1ute_A 43 330 + 288 Gaps:40 95.85 313 31.00 1e-29 mol:protein length:313 PROTEIN (II PURPLE ACID PHOSPHATASE)
blastp_pdb 1war_A 39 321 + 283 Gaps:52 95.16 310 31.19 2e-28 mol:protein length:310 HUMAN PURPLE ACID PHOSPHATASE
blastp_pdb 2bq8_X 39 321 + 283 Gaps:52 97.04 304 31.19 3e-28 mol:protein length:304 TARTRATE-RESISTANT ACID PHOSPHATASE TYPE 5
blastp_pdb 1qfc_A 37 321 + 285 Gaps:48 97.06 306 31.31 1e-27 mol:protein length:306 PROTEIN (PURPLE ACID PHOSPHATASE)
blastp_pdb 1qhw_A 37 321 + 285 Gaps:48 90.83 327 31.31 1e-27 mol:protein length:327 PROTEIN (PURPLE ACID PHOSPHATASE)
blastp_pdb 3tgh_A 43 311 + 269 Gaps:60 83.92 342 28.57 5e-07 mol:protein length:342 Glideosome-associated protein 50
blastp_uniprot_sprot sp|Q8H129|PPA3_ARATH 1 325 + 325 Gaps:2 89.34 366 66.97 5e-166 Purple acid phosphatase 3 OS Arabidopsis thaliana GN PAP3 PE 2 SV 1
blastp_uniprot_sprot sp|Q8VYZ2|PPA8_ARATH 6 327 + 322 Gaps:1 96.42 335 66.87 6e-161 Purple acid phosphatase 8 OS Arabidopsis thaliana GN PAP8 PE 2 SV 1
blastp_uniprot_sprot sp|Q9SCX8|PPA17_ARATH 1 328 + 328 Gaps:7 96.15 338 61.23 6e-152 Purple acid phosphatase 17 OS Arabidopsis thaliana GN PAP17 PE 2 SV 1
blastp_uniprot_sprot sp|Q8VYU7|PPA4_ARATH 28 328 + 301 none 88.79 339 65.45 3e-151 Purple acid phosphatase 4 OS Arabidopsis thaliana GN PAP4 PE 2 SV 1
blastp_uniprot_sprot sp|Q8S341|PPA7_ARATH 9 327 + 319 Gaps:7 98.78 328 60.80 1e-146 Purple acid phosphatase 7 OS Arabidopsis thaliana GN PAP7 PE 2 SV 1
blastp_uniprot_sprot sp|P09889|PPA5_PIG 14 321 + 308 Gaps:40 94.12 340 31.56 3e-30 Tartrate-resistant acid phosphatase type 5 OS Sus scrofa GN ACP5 PE 1 SV 4
blastp_uniprot_sprot sp|P13686|PPA5_HUMAN 36 321 + 286 Gaps:55 92.62 325 31.56 1e-28 Tartrate-resistant acid phosphatase type 5 OS Homo sapiens GN ACP5 PE 1 SV 3
blastp_uniprot_sprot sp|P29288|PPA5_RAT 37 321 + 285 Gaps:48 90.83 327 31.31 5e-27 Tartrate-resistant acid phosphatase type 5 OS Rattus norvegicus GN Acp5 PE 1 SV 1
blastp_uniprot_sprot sp|Q05117|PPA5_MOUSE 37 321 + 285 Gaps:48 90.83 327 30.98 9e-26 Tartrate-resistant acid phosphatase type 5 OS Mus musculus GN Acp5 PE 2 SV 2
blastp_uniprot_sprot sp|O97860|PPA5_RABIT 39 321 + 283 Gaps:44 90.77 325 30.17 2e-25 Tartrate-resistant acid phosphatase type 5 OS Oryctolagus cuniculus GN ACP5 PE 2 SV 1
rpsblast_cdd gnl|CDD|163621 43 316 + 274 Gaps:19 100.00 277 41.16 2e-79 cd07378 MPP_ACP5 Homo sapiens acid phosphatase 5 and related proteins metallophosphatase domain. Acid phosphatase 5 (ACP5) removes the mannose 6-phosphate recognition marker from lysosomal proteins. The exact site of dephosphorylation is not clear. Evidence suggests dephosphorylation may take place in a prelysosomal compartment as well as in the lysosome. ACP5 belongs to the metallophosphatase (MPP) superfamily. MPPs are functionally diverse but all share a conserved domain with an active site consisting of two metal ions (usually manganese iron or zinc) coordinated with octahedral geometry by a cage of histidine aspartate and asparagine residues. The MPP superfamily includes: Mre11/SbcD-like exonucleases Dbr1-like RNA lariat debranching enzymes YfcE-like phosphodiesterases purple acid phosphatases (PAPs) YbbF-like UDP-2 3-diacylglucosamine hydrolases and acid sphingomyelinases (ASMases). The conserved domain is a double beta-sheet sandwich with a di-metal active site made up of residues located at the C-terminal side of the sheets. This domain is thought to allow for productive metal coordination.
rpsblast_cdd gnl|CDD|185607 18 327 + 310 Gaps:59 82.99 394 26.61 3e-20 PTZ00422 PTZ00422 glideosome-associated protein 50 Provisional.
rpsblast_cdd gnl|CDD|201036 45 253 + 209 Gaps:30 98.92 185 19.67 4e-15 pfam00149 Metallophos Calcineurin-like phosphoesterase. This family includes a diverse range of phosphoesterases including protein phosphoserine phosphatases nucleotidases sphingomyelin phosphodiesterases and 2'-3' cAMP phosphodiesterases as well as nucleases such as bacterial SbcD or yeast MRE11. The most conserved regions in this superfamily centre around the metal chelating residues.
rpsblast_kog gnl|CDD|37890 1 327 + 327 Gaps:3 98.21 336 65.76 1e-134 KOG2679 KOG2679 KOG2679 Purple (tartrate-resistant) acid phosphatase [Posttranslational modification protein turnover chaperones].

10 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
PANTHER 9 322 314 PTHR10161 none none none
Phobius 31 332 302 NON_CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. none
SUPERFAMILY 42 326 285 SSF56300 none none IPR029052
Gene3D 38 327 290 G3DSA:3.60.21.10 none none IPR029052
PIRSF 5 332 328 PIRSF000898 "KEGG:00627+3.1.3.2","KEGG:00740+3.1.3.2","MetaCyc:PWY-6348","MetaCyc:PWY-6357","MetaCyc:PWY-6907","MetaCyc:PWY-6908" none IPR024927
Pfam 44 253 210 PF00149 none Calcineurin-like phosphoesterase IPR004843
Phobius 1 30 30 SIGNAL_PEPTIDE none Signal peptide region none
Phobius 10 25 16 SIGNAL_PEPTIDE_H_REGION none Hydrophobic region of a signal peptide. none
Phobius 26 30 5 SIGNAL_PEPTIDE_C_REGION none C-terminal region of a signal peptide. none
Phobius 1 9 9 SIGNAL_PEPTIDE_N_REGION none N-terminal region of a signal peptide. none

3 Localization

Analysis Start End Length
TMHMM 9 28 19
SignalP_GRAM_POSITIVE 1 28 27
SignalP_EUK 1 28 27

5 Qtllist

Qtl Name Chromosome Name Linkage Group Prox Marker Dist Marker Position QTL Pos One Pos Two Test Type Test Value R 2
Bourran_2000_2002_QTL6_Delta.F Qrob_Chr03 3 s_2HYXJ2_207 s_1AUAXK_317 29.12 6,3 45,37 lod 3.1666 0.026
Bourran2_2014_nLBD_3P Qrob_Chr03 3 s_1ET7H8_604 s_1BYNB5_834 23,73 0 46,72 lod 1,7573 3,8
PM_1999_QTL14_peak_Bud_burst_3P Qrob_Chr03 3 s_2F0SI1_756 v_6056_735 11,78 3,78 50,78 lod 2,5 4,5
Bourran2_2014_nFork*_3P Qrob_Chr06 6 v_12930_125 s_1AMZEI_909 34,28 7,43 41,48 lod 2,4044 5,5
Bourran2_2014_rEpiBC*_3P Qrob_Chr03 3 s_1A3B3X_1163 s_1DKGMO_450 23,04 4,92 41,12 lod 2,5668 7

1 Targeting

Analysis Start End Length Location Reliability Signal Peptide Cut Off Mitochondrion Cut Off Network Signal Peptide Length
TargetP 1 28   Secretory pathway 1 0.984 0.009 NON-PLANT 28