Protein : Qrob_P0040720.2 Q. robur
Protein Identifier  ? Qrob_P0040720.2 Organism . Name  Quercus robur
Score  100.0 Score Type  egn
Protein Description  (M=1) K17866 - diphthamide biosynthesis protein 2 Gene Prediction Quality  validated
Protein length 

Sequence

Length: 509  
Kegg Orthology  K17866
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0 Synonyms

1 GO Terms

Identifier Name Description
GO:0017183 peptidyl-diphthamide biosynthetic process from peptidyl-histidine The modification of peptidyl-histidine to 2'-(3-carboxamido-3-(trimethylammonio)propyl)-L-histidine, known as diphthamide, found in translation elongation factor EF-2. The process occurs in eukaryotes and archaea but not eubacteria.

30 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|pper:PRUPE_ppa004543mg 1 506 + 506 Gaps:5 99.80 504 71.77 0.0 hypothetical protein
blastp_kegg lcl|pxb:103957734 1 506 + 506 Gaps:5 99.80 504 70.97 0.0 diphthamide biosynthesis protein 2-like
blastp_kegg lcl|pxb:103957701 1 506 + 506 Gaps:5 99.80 504 70.97 0.0 diphthamide biosynthesis protein 2
blastp_kegg lcl|pmum:103323579 1 506 + 506 Gaps:5 99.80 504 71.37 0.0 diphthamide biosynthesis protein 2
blastp_kegg lcl|vvi:100262956 1 504 + 504 Gaps:8 99.60 506 73.41 0.0 diphthamide biosynthesis protein 2-like
blastp_kegg lcl|mdm:103427470 1 506 + 506 Gaps:5 99.80 504 70.58 0.0 diphthamide biosynthesis protein 2-like
blastp_kegg lcl|cam:101501182 1 504 + 504 Gaps:3 100.00 501 70.26 0.0 diphthamide biosynthesis protein 2-like
blastp_kegg lcl|rcu:RCOM_0547500 1 504 + 504 Gaps:9 100.00 499 70.94 0.0 diphteria toxin resistance protein 2 dph2 putative
blastp_kegg lcl|tcc:TCM_012451 1 505 + 505 Gaps:20 58.70 833 73.42 0.0 Diphthamide synthesis DPH2 family protein
blastp_kegg lcl|pvu:PHAVU_005G177500g 1 504 + 504 Gaps:12 99.80 501 70.00 0.0 hypothetical protein
blastp_pdb 3lzd_B 2 160 + 159 Gaps:13 44.44 378 23.81 9e-06 mol:protein length:378 Dph2
blastp_pdb 3lzd_A 2 160 + 159 Gaps:13 44.44 378 23.81 9e-06 mol:protein length:378 Dph2
blastp_pdb 3lzc_B 2 160 + 159 Gaps:13 44.44 378 23.81 9e-06 mol:protein length:378 Dph2
blastp_pdb 3lzc_A 2 160 + 159 Gaps:13 44.44 378 23.81 9e-06 mol:protein length:378 Dph2
blastp_uniprot_sprot sp|A4QN59|DPH2_DANRE 2 500 + 499 Gaps:63 92.37 498 37.17 1e-85 Diphthamide biosynthesis protein 2 OS Danio rerio GN dph2 PE 2 SV 1
blastp_uniprot_sprot sp|A7SKJ3|DPH2_NEMVE 7 500 + 494 Gaps:50 91.53 531 35.60 8e-85 Diphthamide biosynthesis protein 2 OS Nematostella vectensis GN dph2 PE 3 SV 1
blastp_uniprot_sprot sp|Q5ZKI2|DPH2_CHICK 2 504 + 503 Gaps:55 94.34 477 36.67 1e-76 Diphthamide biosynthesis protein 2 OS Gallus gallus GN DPH2 PE 2 SV 1
blastp_uniprot_sprot sp|Q10206|DPH2_SCHPO 2 505 + 504 Gaps:71 92.45 503 33.12 2e-75 Diphthamide biosynthesis protein 2 OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN dph2 PE 3 SV 1
blastp_uniprot_sprot sp|Q6DE00|DPH2_XENLA 2 500 + 499 Gaps:77 92.89 478 36.04 1e-71 Diphthamide biosynthesis protein 2 OS Xenopus laevis GN dph2 PE 2 SV 1
blastp_uniprot_sprot sp|P0CN20|DPH2_CRYNJ 1 501 + 501 Gaps:60 92.62 515 30.40 2e-59 Diphthamide biosynthesis protein 2 OS Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) GN DPH2 PE 3 SV 1
blastp_uniprot_sprot sp|P0CN21|DPH2_CRYNB 1 501 + 501 Gaps:60 92.62 515 30.40 2e-59 Diphthamide biosynthesis protein 2 OS Cryptococcus neoformans var. neoformans serotype D (strain B-3501A) GN DPH2 PE 3 SV 1
blastp_uniprot_sprot sp|Q568Y2|DPH2_RAT 2 502 + 501 Gaps:74 94.27 489 33.19 6e-51 Diphthamide biosynthesis protein 2 OS Rattus norvegicus GN Dph2 PE 2 SV 1
blastp_uniprot_sprot sp|Q5B2Q1|DPH2_EMENI 2 398 + 397 Gaps:75 66.32 582 32.64 2e-49 Diphthamide biosynthesis protein 2 OS Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) GN dph2 PE 3 SV 1
blastp_uniprot_sprot sp|Q002B5|DPH2_CRIGR 2 502 + 501 Gaps:72 94.27 489 31.67 3e-49 Diphthamide biosynthesis protein 2 OS Cricetulus griseus GN DPH2 PE 2 SV 1
rpsblast_cdd gnl|CDD|188040 6 376 + 371 Gaps:55 99.69 319 37.11 2e-91 TIGR00322 diphth2_R diphthamide biosynthesis enzyme Dph1/Dph2 domain. Archaea and Eukaryotes but not Eubacteria share the property of having a covalently modified residue 2'-[3-carboxamido-3-(trimethylammonio)propyl]histidine as a part of a cytosolic protein. The modified His termed diphthamide is part of translation elongation factor EF-2 and is the site for ADP-ribosylation by diphtheria toxin. This model includes both Dph1 and Dph2 from Saccharomyces cerevisiae although only Dph2 is found in the Archaea (see TIGR03682). Dph2 has been shown to act analogously to the radical SAM (rSAM) family (pfam04055) with 4Fe-4S-assisted cleavage of S-adenosylmethionine to create a free radical but a different organic radical than in rSAM.
rpsblast_cdd gnl|CDD|161796 2 497 + 496 Gaps:60 93.15 496 31.82 3e-69 TIGR00272 DPH2 diphthamide biosynthesis protein 2. This protein has been shown in Saccharomyces cerevisiae to be one of several required for the modification of a particular histidine residue of translation elongation factor 2 to diphthamide. This modified site can then become the target for ADP-ribosylation by diphtheria toxin.
rpsblast_cdd gnl|CDD|190143 28 390 + 363 Gaps:66 99.67 300 30.77 2e-54 pfam01866 Diphthamide_syn Putative diphthamide synthesis protein. Human DPH1 is a candidate tumour suppressor gene. DPH2 from yeast which confers resistance to diphtheria toxin has been found to be involved in diphthamide synthesis. Diphtheria toxin inhibits eukaryotic protein synthesis by ADP-ribosylating diphthamide a posttranslationally modified histidine residue present in EF2. The exact function of the members of this family is unknown.
rpsblast_cdd gnl|CDD|31922 6 390 + 385 Gaps:63 93.95 347 26.38 8e-48 COG1736 DPH2 Diphthamide synthase subunit DPH2 [Translation ribosomal structure and biogenesis].
rpsblast_cdd gnl|CDD|188369 23 389 + 367 Gaps:28 74.35 308 27.51 2e-11 TIGR03682 arCOG04112 diphthamide biosynthesis enzyme Dph2. Members of this family are the archaeal protein Dph2 members of the universal archaeal protein family designated arCOG04112. The chemical function of this protein is analogous to the radical SAM family (pfam04055) although the sequence is not homologous. The chemistry involves [4Fe-4S]-aided formation of a 3-amino-3-carboxypropyl radical rather than the canonical 5'-deoxyadenosyl radical of the radical SAM family.
rpsblast_kog gnl|CDD|37859 6 500 + 495 Gaps:80 92.49 453 38.90 1e-101 KOG2648 KOG2648 KOG2648 Diphthamide biosynthesis protein [Translation ribosomal structure and biogenesis].

6 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
TIGRFAM 2 498 497 TIGR00272 "UniPathway:UPA00559" DPH2: diphthamide biosynthesis protein 2 IPR010014
TIGRFAM 6 376 371 TIGR00322 "UniPathway:UPA00559" diphth2_R: diphthamide biosynthesis enzyme Dph1/Dph2 domain IPR016435
PANTHER 1 507 507 PTHR10762:SF2 "UniPathway:UPA00559";signature_desc=DIPHTHAMIDE BIOSYNTHESIS PROTEIN 2 (DPH2 HOMOLOG) none IPR010014
Pfam 210 387 178 PF01866 "UniPathway:UPA00559" Putative diphthamide synthesis protein IPR016435
Pfam 28 164 137 PF01866 "UniPathway:UPA00559" Putative diphthamide synthesis protein IPR016435
PANTHER 1 507 507 PTHR10762 "UniPathway:UPA00559";signature_desc=DIPHTHAMIDE BIOSYNTHESIS PROTEIN none IPR016435

0 Localization

1 Qtllist

Qtl Name Chromosome Name Linkage Group Prox Marker Dist Marker Position QTL Pos One Pos Two Test Type Test Value R 2
Champenoux_2015_nPriLBD_3P Qrob_Chr10 10 v_15000_157 v_15000_310 15,68 15,9 15,91 lod 2.4 5.5

0 Targeting