Protein : Qrob_P0026100.2 Q. robur
Protein Identifier  ? Qrob_P0026100.2 Organism . Name  Quercus robur
Score  100.0 Score Type  egn
Protein Description  (M=3) 3.1.30.1 - Aspergillus nuclease S(1). Code Enzyme  EC:3.1.30.1
Gene Prediction Quality  validated Protein length 

Sequence

Length: 282  
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0 Synonyms

4 GO Terms

Identifier Name Description
GO:0003676 nucleic acid binding Interacting selectively and non-covalently with any nucleic acid.
GO:0016788 hydrolase activity, acting on ester bonds Catalysis of the hydrolysis of any ester bond.
GO:0004519 endonuclease activity Catalysis of the hydrolysis of ester linkages within nucleic acids by creating internal breaks.
GO:0006308 DNA catabolic process The cellular DNA metabolic process resulting in the breakdown of DNA, deoxyribonucleic acid, one of the two main types of nucleic acid, consisting of a long unbranched macromolecule formed from one or two strands of linked deoxyribonucleotides, the 3'-phosphate group of each constituent deoxyribonucleotide being joined in 3',5'-phosphodiester linkage to the 5'-hydroxyl group of the deoxyribose moiety of the next one.

22 Blast

Analysis Hit Start End Strand Length Note Hit Coverage Hit Length Hit Pident E Val Hit Description
blastp_kegg lcl|mdm:103402686 1 280 + 280 Gaps:5 99.65 286 68.42 2e-145 endonuclease 2
blastp_kegg lcl|tcc:TCM_009671 1 280 + 280 Gaps:6 99.65 287 68.53 2e-143 Endonuclease 2 2 isoform 1
blastp_kegg lcl|pper:PRUPE_ppa009550mg 5 280 + 276 Gaps:6 98.26 287 69.50 2e-143 hypothetical protein
blastp_kegg lcl|mdm:103451640 1 280 + 280 Gaps:6 99.65 287 68.53 4e-143 endonuclease 2-like
blastp_kegg lcl|pmum:103341497 5 280 + 276 Gaps:6 93.38 302 68.79 3e-142 endonuclease 2
blastp_kegg lcl|pxb:103947122 1 280 + 280 Gaps:6 99.65 287 68.18 2e-141 endonuclease 2-like
blastp_kegg lcl|pxb:103947679 1 280 + 280 Gaps:5 99.65 286 66.32 5e-139 endonuclease 2-like
blastp_kegg lcl|cam:101495224 1 281 + 281 Gaps:3 100.00 284 65.85 1e-138 endonuclease 2-like
blastp_kegg lcl|fve:101312828 6 280 + 275 Gaps:8 98.26 288 67.14 2e-137 endonuclease 2-like
blastp_kegg lcl|cit:102621066 7 280 + 274 Gaps:7 97.23 289 67.62 2e-137 endonuclease 2-like
blastp_pdb 1ak0_A 24 277 + 254 Gaps:21 96.67 270 33.33 8e-20 mol:protein length:270 P1 NUCLEASE
blastp_uniprot_sprot sp|Q9C9G4|ENDO2_ARATH 8 280 + 273 Gaps:9 96.55 290 63.93 3e-133 Endonuclease 2 OS Arabidopsis thaliana GN ENDO2 PE 1 SV 1
blastp_uniprot_sprot sp|F4JJL0|ENDO4_ARATH 24 280 + 257 Gaps:9 88.96 299 54.89 1e-102 Endonuclease 4 OS Arabidopsis thaliana GN ENDO4 PE 3 SV 1
blastp_uniprot_sprot sp|Q8LDW6|ENDO3_ARATH 5 280 + 276 Gaps:14 95.24 294 51.07 4e-94 Endonuclease 3 OS Arabidopsis thaliana GN ENDO3 PE 2 SV 1
blastp_uniprot_sprot sp|F4JJL3|ENDO5_ARATH 5 280 + 276 Gaps:10 96.62 296 49.30 2e-93 Endonuclease 5 OS Arabidopsis thaliana GN ENDO5 PE 2 SV 1
blastp_uniprot_sprot sp|Q9SXA6|ENDO1_ARATH 21 280 + 260 Gaps:10 87.87 305 50.00 2e-89 Endonuclease 1 OS Arabidopsis thaliana GN ENDO1 PE 1 SV 1
blastp_uniprot_sprot sp|P24021|NUS1_ASPOR 23 277 + 255 Gaps:18 90.94 287 33.72 4e-31 Nuclease S1 OS Aspergillus oryzae (strain ATCC 42149 / RIB 40) GN nucS PE 1 SV 2
blastp_uniprot_sprot sp|P24504|NUP3_PENSQ 24 277 + 254 Gaps:21 96.67 270 33.33 1e-19 Nuclease PA3 OS Penicillium sp. PE 1 SV 1
blastp_uniprot_sprot sp|P24289|NUP1_PENCI 24 277 + 254 Gaps:21 96.67 270 33.33 3e-19 Nuclease P1 OS Penicillium citrinum PE 1 SV 1
rpsblast_cdd gnl|CDD|202182 24 281 + 258 Gaps:15 100.00 251 47.41 7e-87 pfam02265 S1-P1_nuclease S1/P1 Nuclease. This family contains both S1 and P1 nucleases (EC:3.1.30.1) which cleave RNA and single stranded DNA with no base specificity.
rpsblast_cdd gnl|CDD|211382 24 280 + 257 Gaps:20 100.00 249 39.36 5e-67 cd11010 S1-P1_nuclease S1/P1 nucleases and related enzymes. This family summarizes both S1 and P1 nucleases (EC:3.1.30.1) which cleave RNA and single stranded DNA with no base specificity. S1 nuclease is more active on DNA than RNA. Its reaction products are oligonucleotides or single nucleotides with 5' phosphoryl groups. Although its primary substrate is single-stranded it may also introduce single-stranded breaks in double-stranded DNA or RNA or DNA-RNA hybrids. It is used as a reagent in nuclease protection assays and in removing single stranded tails from DNA molecules to create blunt ended molecules and opening hairpin loops generated during synthesis of double stranded cDNA. P1 nuclease cleaves its substrate at every position yielding nucleoside 5' monophosphates and it does not recognize or act on double-stranded DNA. It is useful at removing single stranded strands hanging off the end of double stranded DNA and at completely cleaving melted DNA for simple DNA composition analysis.
rpsblast_cdd gnl|CDD|211380 66 203 + 138 Gaps:18 58.82 238 21.43 2e-07 cd10981 ZnPC_S1P1 Zinc dependent phospholipase C/S1-P1 nuclease. This model describes both the bacterial and archeal zinc-dependent phospholipase C a domain found in the alpha toxin of Clostridium perfringens as well as S1/P1 nucleases which predominantly act on single-stranded DNA and RNA.

8 Domain Motifs

Analysis Begin End Length Domain Identifier Cross Ref Description Inter Pro
Gene3D 24 281 258 G3DSA:1.10.575.10 none none IPR008947
Pfam 24 281 258 PF02265 none S1/P1 Nuclease IPR003154
Phobius 6 18 13 SIGNAL_PEPTIDE_H_REGION none Hydrophobic region of a signal peptide. none
Phobius 24 281 258 NON_CYTOPLASMIC_DOMAIN none Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. none
Phobius 19 23 5 SIGNAL_PEPTIDE_C_REGION none C-terminal region of a signal peptide. none
SUPERFAMILY 24 278 255 SSF48537 none none IPR008947
Phobius 1 5 5 SIGNAL_PEPTIDE_N_REGION none N-terminal region of a signal peptide. none
Phobius 1 23 23 SIGNAL_PEPTIDE none Signal peptide region none

1 Localization

Analysis Start End Length
SignalP_EUK 1 23 22

0 Qtllist

1 Targeting

Analysis Start End Length Location Reliability Signal Peptide Cut Off Mitochondrion Cut Off Network Signal Peptide Length
TargetP 1 23   Secretory pathway 1 0.976 0.013 NON-PLANT 23